Application
Immunohistochemistry staining of TLR9 in lung tissue using TLR9 Monoclonal Antibody.Application
Western Blot analysis of TLR9 in A) human PBMC, B) human intestine , C) mouse intestine , and D) rat intestine tissue lysates using TLR9 monoclonal antibody (3μ/ml). Lane E shows TLR9 monoclonal antibody tested at 5μ/ml in human MCF7 cell lysate.| Product Name | TLR9 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TLR9, CD289, CD289 antigen, Scri2a, Toll-like receptor 9 |
| Product description | The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. By using a BLAST search, Hemmi et al., 2000 have identified and subsequently isolated a cDNA coding for TLR9. Gene knockout experiments suggest that TLR9 acts as a receptor for unmethylated CpG dinucleotides in the bacterial DNA. Human and mouse TLR9 share an overall amino-acid identity of 75.5%. TLR9 is highly expressed in spleen. |
| Immunogen | TLR9 monoclonal antibody was raised against amino acids 268 - 284 of TLR9 (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1, k |
| Host Species | Mouse |
| Tested Applications | WB |
| TLR9 antibody can be used in Western Blot starting at 0.5 - 2 μg/mL, immunohistochemistry starting at 5 μg/mL, and immunoprecipitation.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | TLR9 |
|---|---|
| Alternative Names | TLR9 CD289 CD289 antigen Scri2a Toll-like receptor 9 |
Application
Immunohistochemistry staining of TLR9 in lung tissue using TLR9 Monoclonal Antibody.
Application
Western Blot analysis of TLR9 in A) human PBMC, B) human intestine , C) mouse intestine , and D) rat intestine tissue lysates using TLR9 monoclonal antibody (3μ/ml). Lane E shows TLR9 monoclonal antibody tested at 5μ/ml in human MCF7 cell lysate.| Application Notes | TLR9 antibody can be used in Western Blot starting at 0.5 - 2 μg/mL, immunohistochemistry starting at 5 μg/mL, and immunoprecipitation.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store TLR9 antibody at 4 ˚C or -20 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | PBS, 0.05% BSA, 0.05% sodium azide. |
