Application
Immunocytochemistry of TMEM214 in A20 cells with TMEM214 antibody at 5 μg/mL.Application
Immunofluorescence of TMEM214 in A20 cells with TMEM214 antibody at 20 μg/mL.Application
Western blot analysis of TMEM214 in A20 cell lysate with TMEM214 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | TMEM214 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TMEM214 Antibody: Transmembrane protein 214 |
| Product description | TMEM214 Antibody: Transmembrane protein 214 (TMEM214) is a 77kD membrane protein which is widely expressed at high level. The gene encoding this protein maps to chromosome 2, at 2p23.3 TMEM214 interacts with LSM1, an SM-like protein, forming a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing. The detailed function of TMEM214 is still unknown, but a recent study showed that TMEM214 may be used to explore the contribution of human host factors of some infectious diseases such as Dengue fever, West Nile fever and yellow fever. Multiple isoforms of TMEM214 are known to exist.1) Wan D, Gong Y, Qin W, et al. Large-scale cDNA transfection screening for genes related to cancer development and progression. Proc. Natl. Acad. Sci. U S A2004;101:15724-9. |
| Immunogen | TMEM214 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human TMEM214. The immunogen is located within amino acids 70 - 120 of TMEM214. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| TMEM214 antibody can be used for detection of TMEM214 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TMEM214 |
|---|---|
| Alternative Names | TMEM214 Antibody: Transmembrane protein 214 |
| Sequence Similarities | 2) Cat. No. 17-208 - A-20 Cell Slide |
Application
Immunocytochemistry of TMEM214 in A20 cells with TMEM214 antibody at 5 μg/mL.
Application
Immunofluorescence of TMEM214 in A20 cells with TMEM214 antibody at 20 μg/mL.
Application
Western blot analysis of TMEM214 in A20 cell lysate with TMEM214 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1288 - A20 Cell Lysate |
|---|---|
| Application Notes | TMEM214 antibody can be used for detection of TMEM214 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TMEM214 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TMEM214 Antibody is supplied in PBS containing 0.02% sodium azide. |
