Application
Western blot analysis of TNFRSF14 in Raji cell lysate with TNFRSF14 antibody at 2 μg/mL in (A) the absence and (B) the presence of blocking peptide.Application
Immunofluorescence of TNFRSF14 in Raji cells with TNFRSF14 antibody at 20 μg/mL.Application
Western blot analysis of 5 ng of TNFRSF14 with TNFRSF14 antibody at 1 μg/mL.Application
Immunocytochemistry of TNFRSF14 in Raji with TNFRSF14 antibody at 10 μg/mL.| Product Name | TNFRSF14 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TNFRSF14 Antibody: TR2, ATAR, HVEA, HVEM, CD270, LIGHTR, UNQ329/PRO509, Tumor necrosis factor receptor superfamily member 14, Herpes virus entry mediator A, Herpesvirus entry mediator A |
| Product description | TNFRSF14 Antibody: Tumor necrosis factor receptor (TNFR) superfamily members are defined by cysteine-rich domains in their extracellular regions that bind TNF-related ligands that share a common structural homology in their extracellular domain. TNFRSF14 was initially identified as the Herpesvirus entry mediator and upon binding to the herpes simplex virus (HSV) envelope glycoprotein D or either of its natural ligands LIGHT and lymphotoxin alpha (LT), activates the transcription factors NF-κB and AP-1. Activation of this signal transduction pathway in T cells stimulates T cell proliferation and cytokine production, leading to inflammation and enhanced CTL-mediated tumor immunity, suggesting that these proteins may be useful as potential targets for controlling cellular immune responses.1) Watts TH. TNF/TNFR family members in costimulation of T cell responses. Annu. Rev. Immunol. 2005; 23:23-68. |
| Immunogen | TNFRSF14 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human TNFRSF14. The immunogen is located within the last 50 amino acids of TNFRSF14. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| TNFRSF14 antibody can be used for the detection of TNFRSF14 by Western blot at 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TNFRSF14 |
|---|---|
| Alternative Names | TNFRSF14 Antibody: TR2 ATAR HVEA HVEM CD270 LIGHTR UNQ329/PRO509 Tumor necrosis factor receptor superfamily member 14 Herpes virus entry mediator A Herpesvirus entry mediator A |
| Sequence Similarities | 2) Cat. No. 17-007 - Raji Cell Slide |
Application
Western blot analysis of TNFRSF14 in Raji cell lysate with TNFRSF14 antibody at 2 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Application
Immunofluorescence of TNFRSF14 in Raji cells with TNFRSF14 antibody at 20 μg/mL.
Application
Western blot analysis of 5 ng of TNFRSF14 with TNFRSF14 antibody at 1 μg/mL.
Application
Immunocytochemistry of TNFRSF14 in Raji with TNFRSF14 antibody at 10 μg/mL.| Positive Control | 1) Cat. No. 1207 - Raji Cell Lysate |
|---|---|
| Application Notes | TNFRSF14 antibody can be used for the detection of TNFRSF14 by Western blot at 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TNFRSF14 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TNFRSF14 Antibody is supplied in PBS containing 0.02% sodium azide. |
