Application
Western blot analysis of TNFRSF14 in mouse thymus tissue lysate with TNFRSF14 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of TNFRSF14 in Mouse Thymus tissue with TNFRSF14 antibody at 10 μg/mL.Application
Western blot analysis of 125 ng of TNFRSF14 with TNFRSF14 antibody at 1 μg/mL.Application
Immunohistochemistry of TNFRSF14 in mouse thymus tissue with TNFRSF14 antibody at 1 μg/mL.| Product Name | TNFRSF14 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TNFRSF14 Antibody: TR2, ATAR, HVEA, HVEM, CD270, LIGHTR, UNQ329/PRO509, Tumor necrosis factor receptor superfamily member 14, Herpes virus entry mediator A, Herpesvirus entry mediator A |
| Product description | TNFRSF14 Antibody: Tumor necrosis factor receptor (TNFR) superfamily members are defined by cysteine-rich domains in their extracellular regions that bind TNF-related ligands that share a common structural homology in their extracellular domain. TNFRSF14 was initially identified as the Herpesvirus entry mediator and upon binding to the herpes simplex virus (HSV) envelope glycoprotein D or either of its natural ligands LIGHT and lymphotoxin alpha (LT), activates the transcription factors NF-κB and AP-1. Activation of this signal transduction pathway in T cells stimulates T cell proliferation and cytokine production, leading to inflammation and enhanced CTL-mediated tumor immunity, suggesting that these proteins may be useful as potential targets for controlling cellular immune responses.1) Montgomery RI, Warner MS, Lum BJ, et al. Herpes simplex virus-1 entry into cells mediated by a novel member of the TNF/NGF receptor family. Cell 1996; 87:427-36. |
| Immunogen | TNFRSF14 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human TNFRSF14. The immunogen is located within amino acids 20 - 70 of TNFRSF14. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| TNFRSF14 antibody can be used for the detection of TNFRSF14 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 1 μg/mL. For immunofluorescence start at 10 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TNFRSF14 |
|---|---|
| Alternative Names | TNFRSF14 Antibody: TR2 ATAR HVEA HVEM CD270 LIGHTR UNQ329/PRO509 Tumor necrosis factor receptor superfamily member 14 Herpes virus entry mediator A Herpesvirus entry mediator A |
| Molecular Weight(MW) | Predicted: 20, 22, 29, 31 kDa Observed: 23 kDa |
| Tissue Specificity | Multiple isoforms of TNFRSF14 are known to exist. |
Application
Western blot analysis of TNFRSF14 in mouse thymus tissue lysate with TNFRSF14 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of TNFRSF14 in Mouse Thymus tissue with TNFRSF14 antibody at 10 μg/mL.
Application
Western blot analysis of 125 ng of TNFRSF14 with TNFRSF14 antibody at 1 μg/mL.
Application
Immunohistochemistry of TNFRSF14 in mouse thymus tissue with TNFRSF14 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1409 - Mouse Thymus Tissue Lysate |
|---|---|
| Application Notes | TNFRSF14 antibody can be used for the detection of TNFRSF14 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 1 μg/mL. For immunofluorescence start at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TNFRSF14 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TNFRSF14 Antibody is supplied in PBS containing 0.02% sodium azide. |
