WB
Western blot analysis of TNFRSF14 on different lysates with Rabbit anti-TNFRSF14 antibody at 1/1,000 dilution. Lane 1: SK-MEL-28 cell lysate, Lane 2: SK-MEL-28 cell lysate treated with deglycosylation, Lane 3: A549 cell lysate (negative), Lysates/proteins at 20 µg/Lane. Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of SK-MEL-28 (positive) and A549 (negative) labeling TNFRSF14 with Rabbit anti-TNFRSF14 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TNFRSF14 antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.FC
Flow cytometric analysis of SK-MEL-28 (left, positive) and A549 (right, negative) cells labeling TNFRSF14. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | TNFRSF14 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human TNFRSF14 aa 1-223 / 283. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFWB |
| WB:1:1000 ICC/IF:1:50 FC:1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | TNFRSF14 |
|---|---|
| Gene Synonyms | TR2 ATAR HVEA HVEM CD270 LIGHTR |
| Gene Full Name | TNF receptor superfamily member 14 |
| Gene Summary | This gene encodes a member of the TNF (tumor necrosis factor) receptor superfamily. The encoded protein functions in signal transduction pathways that activate inflammatory and inhibitory T-cell immune response. It binds herpes simplex virus (HSV) viral envelope glycoprotein D (gD), mediating its entry into cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014] |
| Alternative Names | HVEML antibody ATAR antibody CD270 antibody CD40 like protein precursor antibody Herpes virus entry mediator A antibody Herpesvirus entry mediator A antibody Herpesvirus entry mediator antibody Herpesvirus entry mediator ligand antibody HveA antibody HVEM antibody HVEML antibody ATAR antibody CD270 antibody CD40 like protein precursor antibody Herpes virus entry mediator A antibody Herpesvirus entry mediator A antibody Herpesvirus entry mediator antibody Herpesvirus entry mediator ligand antibody HveA antibody HVEM antibody HVEM L antibody LIGHT antibody LIGHTR antibody TNFRSF14 antibody TNFSF 14 antibody TNR14_HUMAN antibody TR2 antibody Tumor necrosis factor receptor like gene2 antibody Tumor necrosis factor receptor superfamily member 14 antibody Tumor necrosis factor receptor superfamily member 14 precursor antibody Tumor necrosis factor receptor-like 2 antibody |
| Molecular Weight(MW) | 30 kDa(Observed band size: 40-50 kDa) |
| Cellular Localization | Cell membrane. |
WB
Western blot analysis of TNFRSF14 on different lysates with Rabbit anti-TNFRSF14 antibody at 1/1,000 dilution. Lane 1: SK-MEL-28 cell lysate, Lane 2: SK-MEL-28 cell lysate treated with deglycosylation, Lane 3: A549 cell lysate (negative), Lysates/proteins at 20 µg/Lane. Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of SK-MEL-28 (positive) and A549 (negative) labeling TNFRSF14 with Rabbit anti-TNFRSF14 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TNFRSF14 antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.
FC
Flow cytometric analysis of SK-MEL-28 (left, positive) and A549 (right, negative) cells labeling TNFRSF14. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:1000 ICC/IF:1:50 FC:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
