Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.Application
Antibody used in IHC on Human Muscle at 4.0-8.0 ug/ml.Application
Antibody used in WB on HepG2 at 1.25 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).| Product Name | TPM1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TPM1, CMH3, TMSA, TPM1-alpha, CMD1Y, LVNC9, C15orf13, HTM-alpha |
| Product description | TPM1 is a member of the tropomyosin family of highly conserved, widely distributed actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosin is composed of two alpha-helical chains arranged as a coiled-coil. It is polymerized end to end along the two grooves of actin filaments and provides stability to the filaments. The protein is one type of alpha helical chain that forms the predominant tropomyosin of striated muscle, where it also functions in association with the troponin complex to regulate the calcium-dependent interaction of actin and myosin during muscle contraction.This gene is a member of the tropomyosin family of highly conserved, widely distributed actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosin is composed of two alpha-helical chains arranged as a coiled-coil. It is polymerized end to end along the two grooves of actin filaments and provides stability to the filaments. The encoded protein is one type of alpha helical chain that forms the predominant tropomyosin of striated muscle, where it also functions in association with the troponin complex to regulate the calcium-dependent interaction of actin and myosin during muscle contraction. In smooth muscle and non-muscle cells, alternatively spliced transcript variants encoding a range of isoforms have been described. Mutations in this gene are associated with type 3 familial hypertrophic cardiomyopathy.1) Lin, J.C. (2005) Mol. Cell. Biol. 25 (22), 10111-10121. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TPM1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| TPM1 antibody can be used for detection of TPM1 by ELISA at 1:312500. TPM1 antibody can be used for detection of TPM1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
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| Species Reactivity | DogHumanMouseRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | TPM1 |
|---|---|
| Alternative Names | TPM1 CMH3 TMSA TPM1-alpha CMD1Y LVNC9 C15orf13 HTM-alpha |
| Molecular Weight(MW) | 31 kDa, 33 kDa, 33 kDa, 33 kDa |
Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.
Application
Antibody used in IHC on Human Muscle at 4.0-8.0 ug/ml.
Application
Antibody used in WB on HepG2 at 1.25 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | TPM1 antibody can be used for detection of TPM1 by ELISA at 1:312500. TPM1 antibody can be used for detection of TPM1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store TPM1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
