Application
Antibody used in WB on Human THP-1 at 0.2-1 ug/ml.| Product Name | TPM3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TPM3, FLJ41118, MGC14582, MGC3261, MGC72094, NEM1, OK/SW-cl.5, TM-5, TM3, TM30, TM30nm, TPMsk3, TRK, hscp30, TM5, CFTD, CAPM1, HEL-189, HEL-S-82p |
| Product description | TPM3 is a member of the tropomyosin family of actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosins are dimers of coiled-coil proteins that polymerize end-to-end along the major groove in most actin filaments. They provide stability to the filaments and regulate access of other actin-binding proteins. In muscle cells, they regulate muscle contraction by controlling the binding of myosin heads to the actin filament. Mutations in this gene result in autosomal dominant nemaline myopathy, and oncogenes formed by chromosomal translocations involving this locus are associated with cancer.This gene encodes a member of the tropomyosin family of actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosins are dimers of coiled-coil proteins that polymerize end-to-end along the major groove in most actin filaments. They provide stability to the filaments and regulate access of other actin-binding proteins. In muscle cells, they regulate muscle contraction by controlling the binding of myosin heads to the actin filament. Mutations in this gene result in autosomal dominant nemaline myopathy, and oncogenes formed by chromosomal translocations involving this locus are associated with cancer. Multiple transcript variants encoding different isoforms have been found for this gene.1) Clarke, N.F., (2008) Ann. Neurol. 63 (3), 329-337. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TPM3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| TPM3 antibody can be used for detection of TPM3 by ELISA at 1:312500. TPM3 antibody can be used for detection of TPM3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TPM3 |
|---|---|
| Alternative Names | TPM3 FLJ41118 MGC14582 MGC3261 MGC72094 NEM1 OK/SW-cl.5 TM-5 TM3 TM30 TM30nm TPMsk3 TRK hscp30 TM5 CFTD CAPM1 HEL-189 HEL-S-82p |
| Molecular Weight(MW) | 33 kDa |
Application
Antibody used in WB on Human THP-1 at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1208 - THP-1 Cell Lysate |
|---|---|
| Application Notes | TPM3 antibody can be used for detection of TPM3 by ELISA at 1:312500. TPM3 antibody can be used for detection of TPM3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store TPM3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
