Application
Western Blot at 1:2000 dilution Lane 1: human skeletal muscle lysate Lane 2: human heart lysate Lysates/proteins at 20 ug per lane.| Product Name | TPM3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Tropomyosin alpha-3 chain, Gamma-tropomyosin, Tropomyosin-3, Tropomyosin-5, hTM5, TPM3 |
| Product description | Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.1) Reinach F.C.,et al.Nature 322:648-650(1986). |
| Immunogen | This TPM3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 245-276 amino acids from the human region of human TPM3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:2000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TPM3 |
|---|---|
| Alternative Names | Tropomyosin alpha-3 chain Gamma-tropomyosin Tropomyosin-3 Tropomyosin-5 hTM5 TPM3 |
| Molecular Weight(MW) | 33 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western Blot at 1:2000 dilution Lane 1: human skeletal muscle lysate Lane 2: human heart lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
