Application
Western blot analysis of TRAF2 in human liver tissue lysate with TRAF2 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.Application
Immunofluorescence of TRAF2 in human liver tissue with TRAF2 antibody at 20 μg/mL.Application
Immunohistochemistry of TRAF2 in human liver tissue with TRAF2 antibody at 2.5 μg/mL.| Product Name | TRAF2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TRAF2 Antibody: TRAP, TRAP3, MGC:45012, TNF receptor-associated factor 2, E3 ubiquitin-protein ligase TRAF2 |
| Product description | TRAF2 Antibody: Tumor necrosis factor (TNF) receptor associated factors (TRAFs) were initially discovered as adaptor proteins that link the TNF receptor superfamily to signaling pathways and are thus important regulators of cell death and cellular response to stress. TRAF proteins share a homology region that allows them to bind to cell receptors and other TRAF proteins, causing the activation of different signal cascades depending on the TRAFs involved. For example, TRAF2 and TRAF3 directly bind to the CD40, a NF receptor superfamily member involved in inducing B cell immunity, and are critical for NF-κB activation in mouse B lymphocytes. TRAF2 along with TRAF6 has also been shown to be required for CD40 signaling in nonhemopoietic cells. TRAF2 also interacts with the TRFR superfamily member lymphotoxin-beta receptor (LTbetaR) in association with TRAF3 and the apoptosis inhibitors cIAP1 and Smac.1) Arch RH, Gedrich RW, and Thompson CB. Tumor necrosis factor receptor-associated factors (TRAFs) - a family of adaptor proteins that regulate life and death. Genes Dev.1998; 12:2821-30. |
| Immunogen | TRAF2 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human TRAF2. The immunogen is located within amino acids 180 - 230 of TRAF2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| TRAF2 antibody can be used for the detection of TRAF2 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TRAF2 |
|---|---|
| Alternative Names | TRAF2 Antibody: TRAP TRAP3 MGC:45012 TNF receptor-associated factor 2 E3 ubiquitin-protein ligase TRAF2 |
| Sequence Similarities | 2) Cat. No. 10-201 - Human Liver Tissue Slide |
Application
Western blot analysis of TRAF2 in human liver tissue lysate with TRAF2 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Application
Immunofluorescence of TRAF2 in human liver tissue with TRAF2 antibody at 20 μg/mL.
Application
Immunohistochemistry of TRAF2 in human liver tissue with TRAF2 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1304 - Human Liver Tissue Lysate |
|---|---|
| Application Notes | TRAF2 antibody can be used for the detection of TRAF2 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TRAF2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TRAF2 Antibody is supplied in PBS containing 0.02% sodium azide. |
