| Product Name | TRB-3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Tribbles Homolog 3; Neuronal Cell death-inducible Putative Kinase; SINK; p65-interacting Inhibitor of NF-kappaB |
| Product description | Tribbles homolog 3 (TRB-3) is a pseudokinase, shown to act as a potent negative regulator of PPARgamma, a master regulator of adipocyte differentiation, and to control adipogenesis. It disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. It interacts also with the NF-kappaB transactivator p65 RELA. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. |
| Immunogen | Synthetic peptide corresponding to aa 112-125 (A112RLPPHKHVARPTE125) of human TRB-3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| ELISA: (direct and indirect: 1:2,000-1:5,000). Immunohistochemistry: (paraffin sections(1:500)). Western Blot: (1:2,000-1:5,000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | TRB3 |
|---|---|
| Alternative Names | Tribbles Homolog 3 Neuronal Cell death-inducible Putative Kinase SINK p65-interacting Inhibitor of NF-kappaB |
| Tissue Specificity | Recognizes human TRB-3. Detects a band of ~28kDa by Western blot (predicted 39kDa). |
| Application Notes | ELISA: (direct and indirect: 1:2,000-1:5,000). Immunohistochemistry: (paraffin sections(1:500)). Western Blot: (1:2,000-1:5,000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Stable for at least 6 months after receipt when stored at -20˚C. |
| Storage Buffer | Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives. |
