Application
Western blot analysis of TRB3 in mouse kidney tissue lysate with TRB3 antibody at (A) 1 and (B) 2 μg/ml.Application
Immunofluorescence of TRB3 in mouse liver tissue with TRB3 antibody at 20 μg/mL.Application
Immunohistochemistry of TRB3 in mouse liver tissue with TRB3 antibody at 5 μg/mL.| Product Name | TRB3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Tribbles homolog 3, tribbles pseudokinase 3, TRIB3, NIPK, SINK, SKIP3, C20orf97 |
| Product description | TRB3 was initially identified as a novel kinase-like gene that was overexpressed in multiple human tumors and regulated by hypoxia (1). In the liver, TRB3 binds and inhibits AKT activity, leading to impaired insulin signaling (2), whereas in adipose tissue TRB3 is involved in the regulation of fatty acid oxidation through ubiquitination of Acetyl-CoA carboxylase (3). Endoplasmic reticulum (ER) stress induces TRB3 expression in skeletal muscle, leading to a decrease in insulin signaling and glucose uptake, suggesting that inhibition of TRB3 expression may be a potential therapeutic treatment for managing insulin resistance (4).1) Bowers AJ, Scully S, and Boylan JF. SKIP3, a novel drosophila tribbles ortholog, is overexpressed in human tumors and is regulated by hypoxia. Oncogene 2003; 22:2823-35. |
| Immunogen | TRB3 antibody was raised against a 17 amino acid peptide near the center of human TRB3. The immunogen is located within amino acids 100 - 150 of TRB3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| TRB3 antibody can be used for detection of TRB3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TRB3 |
|---|---|
| Alternative Names | Tribbles homolog 3 tribbles pseudokinase 3 TRIB3 NIPK SINK SKIP3 C20orf97 |
| Molecular Weight(MW) | Predicted: 39 kDa Observed: 39 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (76%) |
| Tissue Specificity | TRB3 antibody is human, mouse and rat reactive. At least two isoforms of TRB3 are known to exist; this antibody will detect both isoforms. |
Application
Western blot analysis of TRB3 in mouse kidney tissue lysate with TRB3 antibody at (A) 1 and (B) 2 μg/ml.
Application
Immunofluorescence of TRB3 in mouse liver tissue with TRB3 antibody at 20 μg/mL.
Application
Immunohistochemistry of TRB3 in mouse liver tissue with TRB3 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1405 - Mouse Kidney Tissue Lysate |
|---|---|
| Application Notes | TRB3 antibody can be used for detection of TRB3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TRB3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | TRB3 antibody is supplied in PBS containing 0.02% sodium azide. |
