Application
Western Blot at 1:2000 dilution + human skeletal muscle lysate Lysates/proteins at 20 ug per lane.Application
Western Blot at 1:2000 dilution + human skeletal muscle lysate Lysates/proteins at 20 ug per lane.Application
Western blot analysis of anti-TRIB1 Pab (AP7726b) in T47D cell line lysate (35ug/lane).| Product Name | TRIB1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Tribbles homolog 1, TRB-1, G-protein-coupled receptor-induced gene 2 protein, GIG-2, SKIP1, TRIB1 ( HGNC:16891 ) |
| Product description | TRIB1 antibody, also called gprotein coupled receptor induced, skip1, gig2, c8fwATP, functions in binding protein binding, protein kinase inhibitor activity, and protein serine/threonine kinase activities. Overexpression of TRIB1 in HeLa cells repressed the basal activity of the IL8 promoter by inhibiting AP1 activity. Overexpression of TRIB1 inhibited oncogenic Ras -driven AP1 activation and MEKK1-mediated AP1 activation. ERK activation was enhanced by TRIB1. Coimmunoprecipitation and yeast 2-hybrid assays showed that MEK1 interacted with both TRIB1 and TRIB3, and MKK4 interacted specifically with TRIB1. Cotransfection of MKK4 enhanced the level of TRIB1, indicating that the TRIB-MAPKK interaction stabilized TRIB1.1) Kiss-Toth, E., et al., J. Biol. Chem. 279(41):42703-42708 (2004). |
| Immunogen | This TRIB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 341-370 amino acids from the C-terminal region of human TRIB1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:2000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | TRIB1 |
|---|---|
| Alternative Names | Tribbles homolog 1 TRB-1 G-protein-coupled receptor-induced gene 2 protein GIG-2 SKIP1 TRIB1 ( HGNC:16891 ) |
| Molecular Weight(MW) | 41 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western Blot at 1:2000 dilution + human skeletal muscle lysate Lysates/proteins at 20 ug per lane.
Application
Western Blot at 1:2000 dilution + human skeletal muscle lysate Lysates/proteins at 20 ug per lane.
Application
Western blot analysis of anti-TRIB1 Pab (AP7726b) in T47D cell line lysate (35ug/lane).| Application Notes | For WB starting dilution is: 1:2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
