WB
Positive WB detected in: Raji whole cell lysate, All lanes: TRIB1 antibody at 4.3µg/ml, Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution.IHC
IHC image of TRIB1 Antibody diluted at 1:500 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.ICC/IF
Immunofluorescence staining of HepG2 cells withTRIB1 Antibody at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Product Name | Rabbit anti-TRIB1 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human Tribbles homolog 1 protein (3-91AA) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:5000 IHC:1:500-1:1000 ICC/IF:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TRIB1 |
|---|---|
| Gene Synonyms | C8FW GIG2 TRB1 GIG-2 SKIP1 TRB-1 |
| Gene Full Name | tribbles pseudokinase 1 |
| Gene Summary | Enables RNA polymerase II-specific DNA-binding transcription factor binding activity; mitogen-activated protein kinase kinase binding activity; and protein kinase inhibitor activity. Involved in several processes, including JNK cascade; negative regulation of lipopolysaccharide-mediated signaling pathway; and regulation of protein kinase activity. Located in cytoplasm and nucleus. [provided by Alliance of Genome Resources, Apr 2025] |
| Alternative Names | C8FW antibody G-protein-coupled receptor-induced gene 2 protein antibody GIG-2 antibody GIG2 antibody SKIP1 antibody TRB-1 antibody TRB1 antibody Trib1 antibody TRIB1_HUMAN antibody Tribbles homolog 1 antibody |
| Molecular Weight(MW) | 42 kDa |
| Cellular Localization | cytoplasm,cytosol,nucleus. |
WB
Positive WB detected in: Raji whole cell lysate, All lanes: TRIB1 antibody at 4.3µg/ml, Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution.
IHC
IHC image of TRIB1 Antibody diluted at 1:500 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
ICC/IF
Immunofluorescence staining of HepG2 cells withTRIB1 Antibody at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:500-1:5000 IHC:1:500-1:1000 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
