WB
Western blot analysis of extracts of various cell lines, using KLF3 antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 60s.ICC/IF
Immunofluorescence analysis of A549 cells using KLF3 antibody .| Product Name | KLF3 polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein of human KLF3(NP_057615.3). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500 - 1:2000 ICC/IF:1:50 - 1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TRIB1 |
|---|---|
| Gene Synonyms | C8FW GIG2 TRB1 GIG-2 SKIP1 TRB-1 |
| Gene Full Name | tribbles pseudokinase 1 |
| Gene Summary | Enables RNA polymerase II-specific DNA-binding transcription factor binding activity; mitogen-activated protein kinase kinase binding activity; and protein kinase inhibitor activity. Involved in several processes, including JNK cascade; negative regulation of lipopolysaccharide-mediated signaling pathway; and regulation of protein kinase activity. Located in cytoplasm and nucleus. [provided by Alliance of Genome Resources, Apr 2025] |
| Molecular Weight(MW) | 164kDa |
| Cellular Localization | Nucleus |
WB
Western blot analysis of extracts of various cell lines, using KLF3 antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 60s.
ICC/IF
Immunofluorescence analysis of A549 cells using KLF3 antibody .| Application Notes | WB:1:500 - 1:2000 ICC/IF:1:50 - 1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
| Storage Buffer | 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2. |
