Application
Western blot analysis of TRIF in human lung lysate with TRIF antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of TRIF in Human Lung cells with TRIF antibody at 10 μg/mL.Application
Immunohistochemistry of TRIF in human lung tissue with TRIF antibody at 10 μg/mL.| Product Name | TRIF Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TRIF Antibody: TRIF, IIAE6, MyD88-3, PRVTIRB, TICAM-1, TRIF, TIR domain-containing adapter molecule 1, Proline-rich, vinculin and TIR domain-containing protein B |
| Product description | TRIF Antibody: TRIF is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs). TLRs are signaling molecules that recognize different pathogen-associated molecular patterns (PAMPs) and serve as an important link between the innate and adaptive immune responses. TRIF, along with other molecules such as TIRP, TIRAP, and MyD88, serves as an adaptor protein to several of the TLR molecules. Following activation of TLR3 and TLR4, TRIF engages the kinase TBK1 and allows its subsequent activation of the interferon regulatory factor (IRF)-3. TRIF is also involved in the activation of TNF receptor associated factor (TRAF)-6, and ultimately the activation of NF-κB.1) O’Neill LAJ, Fitzgerald FA, and Bowie AG. The Toll-IL-1 receptor adaptor family grows to five members. Trends in Imm. 2003; 24:286-9. |
| Immunogen | TRIF antibody was raised against a peptide corresponding to 14 amino acids near the C-terminus of human TRIF. The immunogen is located within the last 50 amino acids of TRIF. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| TRIF antibody can be used for detection of TRIF by Western blot at 1 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TICAM1 |
|---|---|
| Alternative Names | TRIF Antibody: TRIF IIAE6 MyD88-3 PRVTIRB TICAM-1 TRIF TIR domain-containing adapter molecule 1 Proline-rich vinculin and TIR domain-containing protein B |
| Molecular Weight(MW) | Predicted: 60 kDa Observed: 55 kDa |
| Sequence Similarities | 2) Cat. No. 10-101 - Human Lung Tissue Slide |
Application
Western blot analysis of TRIF in human lung lysate with TRIF antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of TRIF in Human Lung cells with TRIF antibody at 10 μg/mL.
Application
Immunohistochemistry of TRIF in human lung tissue with TRIF antibody at 10 μg/mL.| Positive Control | 1) Cat. No. 1302 - Human Lung Tissue Lysate |
|---|---|
| Application Notes | TRIF antibody can be used for detection of TRIF by Western blot at 1 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TRIF antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TRIF Antibody is supplied in PBS containing 0.02% sodium azide. |
