Application
Western blot analysis of TRIM5 alpha expression in (A) human stomach, (B) thymus, and (C) uterus tissue lysate with TRIM5 alpha antibody at 2 μg/ml.Application
Immunofluorescence of TRIM5 alpha in human breast tissue with TRIM5 alpha antibody at 10 μg/mL.| Product Name | TRIM5 alpha Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TRIM5 alpha Antibody: RNF88, TRIM5alpha, RNF88, Tripartite motif-containing protein 5, RING finger protein 88 |
| Product description | TRIM5 alpha Antibody: TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region. TRIM5 has six alternately spliced isoforms, the longest of which is the alpha variant which also contains a carboxy-terminal B30.2 (SPRY) domain. Expression of TRIM5α variants from humans, rhesus monkeys, and African green monkeys enabled resistance to infection by various retroviruses including HIV-1, albeit at differing efficiencies. All TRIM5α variants could inhibit at least two different retroviruses, but not from those viruses isolated from the same species, suggesting that TRIM5α acts as a natural barrier to cross-species retrovirus transmission.1) Reymond A, Meroni G, Fantozzi A, et al. The tripartite motif family identifies cell compartments. EMBO J. 2001; 20:2140-51. |
| Immunogen | TRIM5 alpha antibody was raised against a synthetic peptide corresponding to amino acids near the C-terminus of rhesus monkey TRIM5 alpha. The immunogen is located within the last 50 amino acids of TRIM5 alpha. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| TRIM5 alpha antibody can be used for detection of TRIM5 alpha by Western blot at 2 μg/mL. Antibody can also be used for immunoflourescence starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TRIM5 |
|---|---|
| Alternative Names | TRIM5 alpha Antibody: RNF88 TRIM5alpha RNF88 Tripartite motif-containing protein 5 RING finger protein 88 |
| Molecular Weight(MW) | Predicted: 54 kDa Observed: 55 kDa |
| Sequence Similarities | 4) Cat. No. 10-001 - Human Breast Tissue Slide |
| Tissue Specificity | Anti-TRIM5 alpha is human reactive but should presumably also react with rhesus protein. |
Application
Western blot analysis of TRIM5 alpha expression in (A) human stomach, (B) thymus, and (C) uterus tissue lysate with TRIM5 alpha antibody at 2 μg/ml.
Application
Immunofluorescence of TRIM5 alpha in human breast tissue with TRIM5 alpha antibody at 10 μg/mL.| Positive Control | 1) Cat. No. 1319 - Human Stomach Tissue Lysate |
|---|---|
| Application Notes | TRIM5 alpha antibody can be used for detection of TRIM5 alpha by Western blot at 2 μg/mL. Antibody can also be used for immunoflourescence starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TRIM5 alpha antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TRIM5 alpha Antibody is supplied in PBS containing 0.02% sodium azide. |
