Application
Western blot analysis of TRIM5 gamma expression in human bladder (A) and colon (B) cell lysate with TRIM5 gamma antibody at 2 μg /ml.Application
Immunofluorescence of TRIM5 gamma in human bladder tissue with TRIM5 gamma antibody at 20 μg/mL.Application
Immunohistochemistry of TRIM5 gamma in human bladder tissue with TRIM5 gamma antibody at 2 μg/mL.| Product Name | TRIM5 gamma Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TRIM5 gamma Antibody: RNF88, TRIM5alpha, RNF88, Tripartite motif-containing protein 5, RING finger protein 88 |
| Product description | TRIM5 gamma Antibody: TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region. TRIM5 has five alternately spliced isoforms, the longest of which is the alpha variant which also contains a carboxy-terminal B30.2 (SPRY) domain. While a function has not yet been assigned to TRIM5gamma, it is known that expression of TRIM5α variants from humans, rhesus monkeys, and African green monkeys enable resistance to infection by various retroviruses including HIV-1, albeit at differing efficiencies. Furthermore, the TRIM5δ isoform appears to serve as a scaffold for the assembly of endogenous BTBD1 and BTBD2 proteins and also exhibits autoubiquitination activity in a RING finger- and UbcH5B-dependent manner.1) Reymond A, Meroni G, Fantozzi A, et al. The tripartite motif family identifies cell compartments. EMBO J. 2001; 20:2140-51. |
| Immunogen | TRIM5 gamma antibody was raised against a synthetic peptide corresponding to 15 amino acids near the carboxy terminus of human TRIM5 gamma. The immunogen is located within the last 50 amino acids of TRIM5 gamma. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF |
| TRIM5 gamma antibody can be used for detection of TRIM5 gamma by Western blot at 2 μg/mL. A band at approximately 35 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TRIM5 |
|---|---|
| Alternative Names | TRIM5 gamma Antibody: RNF88 TRIM5alpha RNF88 Tripartite motif-containing protein 5 RING finger protein 88 |
Application
Western blot analysis of TRIM5 gamma expression in human bladder (A) and colon (B) cell lysate with TRIM5 gamma antibody at 2 μg /ml.
Application
Immunofluorescence of TRIM5 gamma in human bladder tissue with TRIM5 gamma antibody at 20 μg/mL.
Application
Immunohistochemistry of TRIM5 gamma in human bladder tissue with TRIM5 gamma antibody at 2 μg/mL.| Positive Control | 1) Cat. No. 1310 - Human Bladder Tissue Lysate |
|---|---|
| Application Notes | TRIM5 gamma antibody can be used for detection of TRIM5 gamma by Western blot at 2 μg/mL. A band at approximately 35 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TRIM5 gamma antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TRIM5 gamma Antibody is supplied in PBS containing 0.02% sodium azide. |
