Application
Western blot analysis of TRIM5 delta in human kidney tissue lysate with TRIM5 delta antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.Application
Immunofluorescence of TRIM5 delta in mouse brain tissue with TRIM5 delta antibody at 10 μg/mL.Application
Immunohistochemistry of TRIM5 delta in mouse brain tissue with TRIM5 delta antibody at 1 μg/mL.| Product Name | TRIM5 delta Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TRIM5 delta Antibody: RNF88, TRIM5alpha, RNF88, Tripartite motif-containing protein 5, RING finger protein 88 |
| Product description | TRIM5 delta Antibody: TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region. TRIM5 has five alternately spliced isoforms, the longest of which is the alpha variant which also contains a carboxy-terminal B30.2 (SPRY) domain. Expression of TRIM5α variants from humans, rhesus monkeys, and African green monkeys enabled resistance to infection by various retroviruses including HIV-1, albeit at differing efficiencies. The TRIM5δ isoform has been shown to colocalize with the topoisomerase I-interacting proteins BTBD1 and BTBD2 in punctate or elongated cytoplasmic bodies in several mouse and human cells where it appears to serve as a scaffold for the assembly of endogenous BTBD proteins. TRIM5δ also exhibits autoubiquitination activity in a RING finger- and UbcH5B-dependent manner.1) Reymond A, Meroni G, Fantozzi A, et al. The tripartite motif family identifies cell compartments. EMBO J. 2001; 20:2140-51. |
| Immunogen | TRIM5 delta antibody was raised against a synthetic peptide corresponding to 13 amino acids near the carboxy terminus of human TRIM5 delta. The immunogen is located within the last 50 amino acids of TRIM5 delta. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| TRIM5 delta antibody can be used for detection of TRIM5 delta by Western blot at 2 μg/mL. A band at approximately 25 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 1 μg/mL. For immunofluorescence start at 10 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TRIM5 |
|---|---|
| Alternative Names | TRIM5 delta Antibody: RNF88 TRIM5alpha RNF88 Tripartite motif-containing protein 5 RING finger protein 88 |
| Molecular Weight(MW) | Predicted: 36 kDa Observed: 33 kDa |
| Sequence Similarities | 2) Cat. No. 1403 - Mouse Brain Tissue Lysate |
Application
Western blot analysis of TRIM5 delta in human kidney tissue lysate with TRIM5 delta antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Application
Immunofluorescence of TRIM5 delta in mouse brain tissue with TRIM5 delta antibody at 10 μg/mL.
Application
Immunohistochemistry of TRIM5 delta in mouse brain tissue with TRIM5 delta antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1305 - Human Kidney Tissue Lysate |
|---|---|
| Application Notes | TRIM5 delta antibody can be used for detection of TRIM5 delta by Western blot at 2 μg/mL. A band at approximately 25 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 1 μg/mL. For immunofluorescence start at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TRIM5 delta antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TRIM5 delta Antibody is supplied in PBS containing 0.02% sodium azide. |
