Application
Western blot analysis using Trim5a monoclonal antibody against truncated Trim5αrecombinant protein.Application
Western blot analysis using Trim5a monoclonal antibody against breast tissue lysate with Pierce SuperSignal Febto substrate (top). Immunohistochemical analysis of paraffin - embedded human metastatic adenocarcinoma (middle) and stomach adenocarcinoma (bottom) showing cytoplasmic localization using Trim5a antibody with DAB staining.| Product Name | Trim5a Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | C20orf99C20orf99 |
| Product description | TRIM5-alpha is a protein that is found in the cells of many mammals and fends of various retrovirus infections. It protects monkeys from infection with HIV-1, and humans from infection with some other viruses. If a retrovirus has entered a cell, it needs to shed its capsid in order to reversely transcribe its genes, so that they can be expressed by the host cell. It is believed that TRIM5α, which is present in the cytoplasm, somehow recognizes the capsid and blocks its shedding, thereby stopping the virus in its tracks. It thus represents an intracellular defense completely separate from the rest of the body's immune system.1) Stremlau, M. Nature 2004.427:848-53. |
| Immunogen | Ni-NTA purified truncated recombinant human trim5 alpha expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| Western Blot:Dilution 1:1,000 - 1:5,000 IHC(P):Dilution 1:100 - 1:400 IHC(F):Dilution 1:200 - 1:400 Determining optimal working dilutions by titration test.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | ZCCHC3 |
|---|---|
| Alternative Names | C20orf99C20orf99 |
Application
Western blot analysis using Trim5a monoclonal antibody against truncated Trim5αrecombinant protein.
Application
Western blot analysis using Trim5a monoclonal antibody against breast tissue lysate with Pierce SuperSignal Febto substrate (top). Immunohistochemical analysis of paraffin - embedded human metastatic adenocarcinoma (middle) and stomach adenocarcinoma (bottom) showing cytoplasmic localization using Trim5a antibody with DAB staining.| Application Notes | Western Blot:Dilution 1:1,000 - 1:5,000 IHC(P):Dilution 1:100 - 1:400 IHC(F):Dilution 1:200 - 1:400 Determining optimal working dilutions by titration test.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Trim5α monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
