Application
Western blot analysis of TSLP in A-20 cell lysate with TSLP antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.Application
Immunofluorescence of TSLP in Mouse Brain tissue with TSLP antibody at 20 μg/mL.Application
Immunohistochemistry of TSLP in mouse brain tissue with TSLP antibody at 2.5 μg/mL.| Product Name | TSLP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TSLP Antibody: Thymic stromal lymphopoietin |
| Product description | TSLP Antibody: Thymic stromal lymphopoietin (TSLP) has recently been identified as an important factor capable of driving dendritic cell maturation and activation. TSLP is a four-helix-bundle cytokine that is expressed mainly by barrier epithelial cells and is a potent activator of several cell types such as myeloid dendritic cells. TSLP is involved in the positive selection of regulatory T cells, maintenance of peripheral CD4+ T cell homeostasis and the induction of CD4+ T cell-mediated allergic reaction. TSLP is also capable of supporting the growth of fetal liver and adult B cell progenitors and their differentiation to the IgM-positive stage of B cell development. Amino acid sequence analysis has shown poor homology between human and mouse TSLP although they exhibit similar biological functions and are expressed in similar tissues. At least two differentially spliced isoforms of TSLP are known to exist.1) Ziegler SF and Liu Y-J. Thymic stromal lymphopoietin in normal and pathogenic T cell development and function. Nature Immunol. 2006; 7:709-14. |
| Immunogen | TSLP antibody was raised against a 20 amino acid synthetic peptide from near the center of mouse TSLP. The immunogen is located within amino acids 70 - 120 of TSLP. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| TSLP antibody can be used for detection of TSLP by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TSLP |
|---|---|
| Alternative Names | TSLP Antibody: Thymic stromal lymphopoietin |
| Molecular Weight(MW) | Predicted: 18 kDa Observed: 24 kDa |
| Sequence Similarities | 2) Cat. No. 1403 - Mouse Brain Tissue Lysate |
Application
Western blot analysis of TSLP in A-20 cell lysate with TSLP antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Application
Immunofluorescence of TSLP in Mouse Brain tissue with TSLP antibody at 20 μg/mL.
Application
Immunohistochemistry of TSLP in mouse brain tissue with TSLP antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1288 - A20 Cell Lysate |
|---|---|
| Application Notes | TSLP antibody can be used for detection of TSLP by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TSLP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TSLP Antibody is supplied in PBS containing 0.02% sodium azide. |
