Application
Tyrosine Hydroxylase (Phospho-Ser19) Antibody| Product Name | Tyrosine Hydroxylase (phospho Ser19) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | The, Tyrosine 3-hydroxylase, TH |
| Product description | Tyrosine Hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines Dopamine and Norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons. We raised this polyclonal antibody against a peptide representing the sequence around Ser19 in rat TH purified. This antibody is suitable for most immunochemical applications in a variety of mammalian and some non-mammalian species.1) Witkovsky, P., Gabriel, R., Haycock, J. W., and Meller, E., "Influence of light and neural circuitry on tyrosine hydroxylase phosphorylation in the rat retina," J. Chem. Neuroanat., 19 (2000) 105 - 116. |
| Immunogen | TH (Ser19) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Ser19 of rat tyrosine hydroxylase (TH). |
| Modification | p-Ser19 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state. Initial recommended range of dilutions: 1:1000 to 1:2000. Applications include Dot Blots (DB), Immunohistochemistry (IHC), Immunofluorescence (IF) and Western Blot (WB). When internally tested under ideal conditions the working dilutions were 1:1000 for IF, IHC, DB and WB.: |
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| Species Reactivity | Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Th |
|---|---|
| Alternative Names | The Tyrosine 3-hydroxylase TH |
| Molecular Weight(MW) | 60 |
| Tissue Specificity | Tyrosine Hydroxylase antibody is specific for the 60k Tyrosine Hydroxylase phosphorylated at Ser19 in PC-12 cells. |
Application
Tyrosine Hydroxylase (Phospho-Ser19) Antibody| Application Notes | Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state. Initial recommended range of dilutions: 1:1000 to 1:2000. Applications include Dot Blots (DB), Immunohistochemistry (IHC), Immunofluorescence (IF) and Western Blot (WB). When internally tested under ideal conditions the working dilutions were 1:1000 for IF, IHC, DB and WB.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol. |
