Application
Antibody used in IHC on Human kidney.Application
Antibody used in WB on recombinant protein at: 1:500 (Lanes: 1: 40ng HIS-UBE2D1 protein, 2: 40ng HIS-UBE2D2 protein, 3: 40ng HIS-UBE2D3 proteinm, 4: 40ng HIS-UBE2D4 protein, 5: 40ng HIS-UBE2E1 protein, 6: 40ng HIS-UBE2E2 protein, 7: 40ng HIS-UBE2E3 protein, 8: 40ng HIS-UBE2K protein, 9: 40ng HIS-UBE2L3 protein, 10: 40ng HIS-UBE2N protein, 11: 40ng HIS-UBE2V1 protein, 12: 40ng HIS-UBE2V2 protein.).Application
Antibody used in WB on Human Jurkat 0.125 ug/ml.| Product Name | UBE2E2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UBE2E2, FLJ25157, UBCH8 |
| Product description | UBE2E2 catalyzes the covalent attachment of ubiquitin to other proteins.1) Plafker, S.M., (2004) J. Cell Biol. 167 (4), 649-659. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human UBE2E2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| UBE2E2 antibody can be used for detection of UBE2E2 by ELISA at 1:1562500. UBE2E2 antibody can be used for detection of UBE2E2 by western blot at 0.125 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | UBE2E2 |
|---|---|
| Alternative Names | UBE2E2 FLJ25157 UBCH8 |
| Molecular Weight(MW) | 22 kDa |
Application
Antibody used in IHC on Human kidney.
Application
Antibody used in WB on recombinant protein at: 1:500 (Lanes: 1: 40ng HIS-UBE2D1 protein, 2: 40ng HIS-UBE2D2 protein, 3: 40ng HIS-UBE2D3 proteinm, 4: 40ng HIS-UBE2D4 protein, 5: 40ng HIS-UBE2E1 protein, 6: 40ng HIS-UBE2E2 protein, 7: 40ng HIS-UBE2E3 protein, 8: 40ng HIS-UBE2K protein, 9: 40ng HIS-UBE2L3 protein, 10: 40ng HIS-UBE2N protein, 11: 40ng HIS-UBE2V1 protein, 12: 40ng HIS-UBE2V2 protein.).
Application
Antibody used in WB on Human Jurkat 0.125 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | UBE2E2 antibody can be used for detection of UBE2E2 by ELISA at 1:1562500. UBE2E2 antibody can be used for detection of UBE2E2 by western blot at 0.125 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store UBE2E2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
