Application
Western blot analysis in mouse Neuro-2a,Jurkat,Ramos cell line lysates (35ug/lane).Application
Flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Product Name | UBE2E2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Ubiquitin-conjugating enzyme E2 E2, UbcH8, Ubiquitin carrier protein E2, Ubiquitin-protein ligase E2, UBE2E2, UBCH8 |
| Product description | Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination.1) Yamauchi, T., et al. Nat. Genet. 42(10):864-868(2010) |
| Immunogen | This UBE2E2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-43 amino acids from the N-terminal region of human UBE2E2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | UBE2E2 |
|---|---|
| Alternative Names | Ubiquitin-conjugating enzyme E2 E2 UbcH8 Ubiquitin carrier protein E2 Ubiquitin-protein ligase E2 UBE2E2 UBCH8 |
| Molecular Weight(MW) | 22 kDa |
Application
Western blot analysis in mouse Neuro-2a,Jurkat,Ramos cell line lysates (35ug/lane).
Application
Flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
