Application
Western blot analysis in A2058 cell line lysates (35ug/lane).| Product Name | UBE2H Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Ubiquitin-conjugating enzyme E2 H, UbcH2, Ubiquitin carrier protein H, Ubiquitin-conjugating enzyme E2-20K, Ubiquitin-protein ligase H, UBE2H |
| Product description | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and zebrafish homologs. Two alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms.1) Lausen, J., et al. J. Biol. Chem. 285(8):5338-5346(2010) |
| Immunogen | This UBE2H antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 42-70 amino acids from the Central region of human UBE2H. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | UBE2H |
|---|---|
| Alternative Names | Ubiquitin-conjugating enzyme E2 H UbcH2 Ubiquitin carrier protein H Ubiquitin-conjugating enzyme E2-20K Ubiquitin-protein ligase H UBE2H |
| Molecular Weight(MW) | 21 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis in A2058 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
