Application
Antibody is used in Western blot to detect UBE4B in mouse kidney tissue lysate.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | UBE4B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Ubiquitin conjugation factor E4 B, 632-, UBE4B ( HGNC:12500 ) |
| Product description | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE4B represents an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly. UBE4B also the strongest candidate among the neuroblastoma tumor suppressors.1) Ishikawa, K., et al., DNA Res. 5(3):169-176 (1998). |
| Immunogen | This UBE4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 112-142 amino acids from the N-terminal region of human UBE4B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | UBE4B |
|---|---|
| Alternative Names | Ubiquitin conjugation factor E4 B 632- UBE4B ( HGNC:12500 ) |
| Molecular Weight(MW) | 146 kDa |
Application
Antibody is used in Western blot to detect UBE4B in mouse kidney tissue lysate.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
