UCRC is a subunit of mitochondrial complex III (ubiquinol-cytochrome c reductase; EC 1.10.2.2), which forms the middle segment of the respiratory chain of the inner mitochondrial membrane.UCRC is a subunit of mitochondrial complex III (ubiquinol-cytochrome c reductase; EC 1.10.2.2), which forms the middle segment of the respiratory chain of the inner mitochondrial membrane (Schagger et al., 1995 [PubMed 8592474]).1) Guzy, R.D., (2005) Cell Metab. 1 (6), 401-408.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human UCRC.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
UCRC antibody can be used for detection of UCRC by ELISA at 1:62500. UCRC antibody can be used for detection of UCRC by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.
Positive Control
1) Cat. No. 1219 - MCF7 Cell Lysate
Application Notes
UCRC antibody can be used for detection of UCRC by ELISA at 1:62500. UCRC antibody can be used for detection of UCRC by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store UCRC antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.