WB
Western blot analysis of Cytochrome b-c1 complex subunit 9/UQCR10 on different lysates with Rabbit anti-Cytochrome b-c1 complex subunit 9/UQCR10 antibody at 1/5000 dilution. Lane 1: MCF7 cell lysate, Lane 2: HeLa cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 9 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of MCF7 cells labeling Cytochrome b-c1 complex subunit 9/UQCR10 with Rabbit anti-Cytochrome b-c1 complex subunit 9/UQCR10 antibody at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Cytochrome b-c1 complex subunit 9/UQCR10 antibody at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.IHC
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-Cytochrome b-c1 complex subunit 9/UQCR10 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.FC
Flow cytometric analysis of MCF7 cells labeling Cytochrome b-c1 complex subunit 9/UQCR10. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | Cytochrome b-c1 complex subunit 9/UQCR10 Rabbit Polyclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within C-terminal human UQCR10 from aa42-aa63 . |
| Clonality | polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:5000 ICC/IF:1:250 IHC:1:1000 FC:1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | UQCR10 |
|---|---|
| Gene Synonyms | QCR9 UCRC HSPC051 HSPC119 HSPC151 UCCR7.2 |
| Gene Full Name | ubiquinol-cytochrome c reductase, complex III subunit X |
| Gene Summary | UCRC is a subunit of mitochondrial complex III (ubiquinol-cytochrome c reductase; EC 1.10.2.2), which forms the middle segment of the respiratory chain of the inner mitochondrial membrane (Schagger et al., 1995 [PubMed 8592474]).[supplied by OMIM, Mar 2008] |
| Alternative Names | Cytochrome c1 non heme 7 kDa protein antibody Cytochrome C1 nonheme 7kDa protein antibody HSPC051 antibody HSPC119 antibody HSPC151 antibody QCR9 antibody Ubiquinol cytochrome c reductase complex antibody Ubiquinol cytochrome c reductase complex (7.2 kD) antibody Ubiquinol cytochrome c reductase complex 7.2 kDa protein antibody Ubiquinol cytochrome c reductase complex III subunit X 7.2kDa antibody Cytochrome c1 non heme 7 kDa protein antibody Cytochrome C1 nonheme 7kDa protein antibody HSPC051 antibody HSPC119 antibody HSPC151 antibody QCR9 antibody Ubiquinol cytochrome c reductase complex antibody Ubiquinol cytochrome c reductase complex (7.2 kD) antibody Ubiquinol cytochrome c reductase complex 7.2 kDa protein antibody Ubiquinol cytochrome c reductase complex III subunit X 7.2kDa antibody Ubiquinol cytochrome c reductase complex III subunit X antibody UCCR7.2 antibody UCRC antibody UQCR10 antibody |
| Molecular Weight(MW) | 7kDa(Observed band size:7kDa) |
| Cellular Localization | Mitochondrion inner membrane. |
WB
Western blot analysis of Cytochrome b-c1 complex subunit 9/UQCR10 on different lysates with Rabbit anti-Cytochrome b-c1 complex subunit 9/UQCR10 antibody at 1/5000 dilution. Lane 1: MCF7 cell lysate, Lane 2: HeLa cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 9 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of MCF7 cells labeling Cytochrome b-c1 complex subunit 9/UQCR10 with Rabbit anti-Cytochrome b-c1 complex subunit 9/UQCR10 antibody at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Cytochrome b-c1 complex subunit 9/UQCR10 antibody at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.
IHC
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-Cytochrome b-c1 complex subunit 9/UQCR10 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
FC
Flow cytometric analysis of MCF7 cells labeling Cytochrome b-c1 complex subunit 9/UQCR10. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:5000 ICC/IF:1:250 IHC:1:1000 FC:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
