Application
Western blot analysis of ULK1 in rat brain tissue lysate with ULK1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.Application
Immunofluorescence of ULK1 in rat brain tissue with ULK1 antibody at 20 μg/ml.Application
Immunohistochemistry of ULK1 in rat brain tissue with ULK1 antibody at 2.5 μg/ml.| Product Name | ULK1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ULK1 Antibody: ATG1, ATG1A, UNC51, hATG1, Unc51.1, KIAA0722, Autophagy-related protein 1 homolog, ATG1 |
| Product description | ULK1 Antibody: ULK1, also known as ATG1, is a key serine/threonine protein kinase probably acting at the most upstream step of autophagosome formation. Knockout of ULK1 results in a severe defect in the autophagy pathway. ULK1 is highly conserved among eukaryotes, and are the Unc-51-like kinases, ULK1 and ULK2 in mammals. ULK1 is ubiquitously expressed and involved in autophagy in response to starvation. It is the target of the TOR kinase signaling pathway that regulates autophagy through the control of phosphorylation status of ATG13. ULK1 also plays a role early in neuronal differentiation.1) Suzuki K, Kubota Y, Sekito T, et al. Hierarchy of Atg proteins in pre-autophagosomal structure organization. Genes to Cells 2007; 12:209–18. |
| Immunogen | ULK1 antibody was raised against a 16 amino acid peptide near the center of human ULK1 . The immunogen is located within amino acids 350 - 400 of ULK1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| ULK1 Antibody can be used for detection of ULK1 by Western blot at 1 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ULK1 |
|---|---|
| Alternative Names | ULK1 Antibody: ATG1 ATG1A UNC51 hATG1 Unc51.1 KIAA0722 Autophagy-related protein 1 homolog ATG1 |
| Molecular Weight(MW) | Predicted: 116 kDa Observed: 105 kDa |
| Tissue Specificity | Two alternatively spliced transcript variants encoding different isoforms have been identified. ULK1 antibody is predicted to not cross-react with ULK2. |
Application
Western blot analysis of ULK1 in rat brain tissue lysate with ULK1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Application
Immunofluorescence of ULK1 in rat brain tissue with ULK1 antibody at 20 μg/ml.
Application
Immunohistochemistry of ULK1 in rat brain tissue with ULK1 antibody at 2.5 μg/ml.| Positive Control | 1) Cat. No. 1463 - Rat Brain Tissue Lysate |
|---|---|
| Application Notes | ULK1 Antibody can be used for detection of ULK1 by Western blot at 1 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ULK1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | ULK1 Antibody is supplied in PBS containing 0.02% sodium azide. |
