Application
Western blot analysis of extracts from HepG2 cells treated with Na3VO4 using Phospho-VE-Cadherin (Tyr731) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | VE-Cadherin (phospho Tyr731) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Vascular endothelial cadherin, VE-cadherin, 7B4 antigen, CDH5, CD144 antigen |
| Product description | Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen.1) Wessel F, et al. (2014) Nat Immunol 15, 223-30 |
| Immunogen | VE-Cadherin (phospho Tyr731) antibody was raised against a peptide sequence around phosphorylation site of Tyrosine731 (H-I-Y (p)-G-Y) derived from Human VE-Cadherin. |
| Modification | p-Tyr731 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CDH5 |
|---|---|
| Alternative Names | Vascular endothelial cadherin VE-cadherin 7B4 antigen CDH5 CD144 antigen |
| Molecular Weight(MW) | 130 kDa |
| Tissue Specificity | The antibody detects endogenous level of VE-Cadherin only when phosphorylated at tyrosine 731. |
| Entrez Gene | 1003 |
|---|---|
| Protein Accession | NP_001786.2 |
Application
Western blot analysis of extracts from HepG2 cells treated with Na3VO4 using Phospho-VE-Cadherin (Tyr731) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
