Application
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~53k VGAT protein.| Product Name | Vesicular GABA transporter Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Vgat, Viaat, Vgat, GABA and glycine transporter, |
| Product description | The Vesicular GABA Amino Acid Transporter (VGAT) is responsible for transport of the inhibitory neurotransmitter into synaptic vesicles(McIntire et al., 1997). The VGAT protein (also known as the Vesicular Inhibitory Amino Aid Transporter or VIAAT) is expressed in synaptic vesicles of both glycine and GABAergic synapses throughout the CNS (Chaudhry et al., 1998). Expression of the VGAT protein changes during development and also in response to patterns of neuronal activity (De et al., 2005).1) Chaudhry FA, Reimer RJ, Bellocchio EE, Danbolt NC, Osen KK, Edwards RH, Storm-Mathisen J (1998) The vesicular GABA transporter, VGAT, localizes to synaptic vesicles in sets of glycinergic as well as GABAergic neurons. J Neurosci 18:9733-9750. |
| Immunogen | Peptide corresponding to amino acid residues from the N-terminal region of rat Vesicular GABA Amino Acid Transporter (VGAT). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
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| Species Reactivity | BovineDogRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Slc32a1 |
|---|---|
| Alternative Names | Vgat Viaat Vgat GABA and glycine transporter |
| Molecular Weight(MW) | 53 |
Application
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~53k VGAT protein.| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Vesicular GABA transporter antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year. |
| Storage Buffer | 100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol. |
