IF-F
Application: IHC-Fr; Species: Mouse; Site: Cerebellum; Sample: Frozen section; Antibody concentration: 1:500; Antigen retrieval: Not required.IF-F
Application: IHC-Fr; Species: Mouse; Site: Cerebellum; Sample: Frozen section; Antibody concentration: 1:500; Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.IHC
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-SLC32A1 / VGAT antibody at 1/1,000 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-SLC32A1 / VGAT antibody at 1/1,000 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-SLC32A1 / VGAT antibody at 1/1,000 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-SLC32A1 / VGAT antibody at 1/1,000 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | SLC32A1 / VGAT Recombinant Antibody - Rabbit IgG (Chimeric) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Tested Applications | IF-FIHC |
| IHC:1:1000 IF-F:1:500 |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | Slc32a1 |
|---|---|
| Gene Synonyms | VGAT Viaat |
| Gene Full Name | solute carrier family 32 (GABA vesicular transporter), member 1 |
| Gene Summary | Enables gamma-aminobutyric acid:proton antiporter activity and glycine:proton antiporter activity. Involved in gamma-aminobutyric acid import and glycine transport. Located in several cellular components, including cone cell pedicle; dendrite terminus; and inhibitory synapse. Is expressed in several structures, including cardiovascular system; central nervous system; eye; metanephros; and skeleton. Used to study temporal lobe epilepsy. Human ortholog(s) of this gene implicated in developmental and epileptic encephalopathy and generalized epilepsy with febrile seizures plus. Orthologous to human SLC32A1 (solute carrier family 32 member 1). [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | bA122O1.1 antibody GABA and glycine transporter antibody hVIAAT antibody SLC32A 1 antibody Slc32a1 antibody solute carrier family 32 (GABA vesicular transporter) member 1 antibody Solute carrier family 32 member 1 antibody Vesicular GABA Amino Acid Transporter antibody Vesicular GABA transporter antibody Vesicular inhibitory amino acid transporter antibody bA122O1.1 antibody GABA and glycine transporter antibody hVIAAT antibody SLC32A 1 antibody Slc32a1 antibody solute carrier family 32 (GABA vesicular transporter) member 1 antibody Solute carrier family 32 member 1 antibody Vesicular GABA Amino Acid Transporter antibody Vesicular GABA transporter antibody Vesicular inhibitory amino acid transporter antibody VGAT antibody VIAAT antibody VIAAT_HUMAN antibody |
| Molecular Weight(MW) | 57 kDa |
| Cellular Localization | Cytoplasmic vesicle membrane, Presynapse. |
IF-F
Application: IHC-Fr; Species: Mouse; Site: Cerebellum; Sample: Frozen section; Antibody concentration: 1:500; Antigen retrieval: Not required.
IF-F
Application: IHC-Fr; Species: Mouse; Site: Cerebellum; Sample: Frozen section; Antibody concentration: 1:500; Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.
IHC
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-SLC32A1 / VGAT antibody at 1/1,000 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-SLC32A1 / VGAT antibody at 1/1,000 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-SLC32A1 / VGAT antibody at 1/1,000 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-SLC32A1 / VGAT antibody at 1/1,000 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | IHC:1:1000 IF-F:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
