Application
Western blot analysis of Vinculin in 293, A431, A549, HeLa, HepG2, K562, 3T3, Raji, U937 cell lysate and rat heart, mouse lung, rat lung, mouse spleen, rat spleen, rabbit spleen, mouse brain, rabbit brain and chicken spleen tissue lysate with Vinculin antibody at 1 μg/ml.| Product Name | Vinculin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CMD1W, CMH15, Metavinculin, MVCL |
| Product description | Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development (1). Three structural vinculin domains include an amino-terminal head, a short flexible proline-rich region and a carboxy-terminal tail (2). Expression of vinculin were shown to be affected by the level of actin expression (2,3). Vinculin deficiencies are associated with a decrease in cell adhesion and an increase in cell motility, suggesting a possible role in metastatic growth (4). Defects in VCL are the cause of cardiomyopathy dilated type 1W (CMD1W) (5).1) Burridge K, Fath K, Kelly T, et al. Focal adhesions: transmembrane junctions between the extracellular matrix and the cytoskeleton. Annu. Rev. Cell Biol.1988; 4:487-525. |
| Immunogen | Vinculin antibody was raised against a 23 amino acid peptide near the amino terminus of human Vinculin. The immunogen is located within amino acids 50 - 100 of Vinculin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| Vinculin antibody can be used for detection of Vinculin by Western blot at 0.5 - 1 μg/ml.: |
|
| Species Reactivity | ChickenHumanMouseRabbitRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | VCL |
|---|---|
| Alternative Names | CMD1W CMH15 Metavinculin MVCL |
| Molecular Weight(MW) | Predicted: 117 kDa Observed: 120 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Pig: (100%) |
| Tissue Specificity | Vinculin antibody is human, mouse, rat, rabbit and chicken reactive. At least three isoforms of Vinculin are known to exist. |
Application
Western blot analysis of Vinculin in 293, A431, A549, HeLa, HepG2, K562, 3T3, Raji, U937 cell lysate and rat heart, mouse lung, rat lung, mouse spleen, rat spleen, rabbit spleen, mouse brain, rabbit brain and chicken spleen tissue lysate with Vinculin antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1216 - PC-3 Cell Lysate |
|---|---|
| Application Notes | Vinculin antibody can be used for detection of Vinculin by Western blot at 0.5 - 1 μg/ml.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Vinculin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | Vinculin Antibody is supplied in PBS containing 0.02% sodium azide. |
