Application
To detect Human Visfatin by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human Visfatin (38-282) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human Visfatin.Application
To detect Human Visfatin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human Visfatin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect Human Visfatin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human Visfatin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.Application
This antibody stained formalin-fixed, paraffin-embedded sections of human ovary malignant adeoncarcinoma. The recommended concentration is 7.5ng/mL -15ng/mL with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.Application
This antibody stained formalin-fixed, paraffin-embedded sections of human ovary malignant adeoncarcinoma. The recommended concentration is 7.5ng/mL -15ng/mL with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.| Product Name | Visfatin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | VF, PBEF, PBEF1, VISFATIN, 1110035O14Rik, Nicotinamide phosphoribosyltransferase, Pre-B-cell colony-enhancing factor 1, NAmPRTase |
| Immunogen | Produced from sera of rabbits immunized with highly pure recombinant Human Visfatin. Human Visfatin specific antibody was purified by affinity chromatography employing an immobilized Human Visfatin matrix. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELISA: Sandwich: To detect Human Visfatin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human Visfatin (38-282) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human Visfatin. Western Blot: To detect Human Visfatin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human Visfatin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | NAMPT |
|---|---|
| Alternative Names | VF PBEF PBEF1 VISFATIN 1110035O14Rik Nicotinamide phosphoribosyltransferase Pre-B-cell colony-enhancing factor 1 NAmPRTase |
Application
To detect Human Visfatin by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human Visfatin (38-282) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human Visfatin.
Application
To detect Human Visfatin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human Visfatin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect Human Visfatin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human Visfatin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Application
This antibody stained formalin-fixed, paraffin-embedded sections of human ovary malignant adeoncarcinoma. The recommended concentration is 7.5ng/mL -15ng/mL with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
Application
This antibody stained formalin-fixed, paraffin-embedded sections of human ovary malignant adeoncarcinoma. The recommended concentration is 7.5ng/mL -15ng/mL with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.| Application Notes | ELISA: Sandwich: To detect Human Visfatin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human Visfatin (38-282) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human Visfatin. Western Blot: To detect Human Visfatin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human Visfatin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The lyophilized antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-80˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
