Application
Western blot analysis of VTCN1 in EL4 cell lysate with VTCN1 antibody at 1 μg/mL.| Product Name | VTCN1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | VTCN1 Antibody: T cell immunity plays a critical role in host immune surveillance of tumour cell growth and metastatic spread. VTCN1, also known as B7-H4, is a member of the B7 family of immune regulatory molecules. Expressed primarily on the membrane of lymphoid cells, VTCN1 is an immunoinhibitory protein that interacts with receptors on the surface of T lymphocytes, thus mediating cellular and humoral immune responses. Overexpression of VTCN1 is associated with certain malignancies, including ovarian and breast cancer, which may be a mechanism by which tumour cells suppress T cell immunity and facilitate tumour progression. VTCN1 may thus be a useful biomarker for the early detection of ovarian cancer.1) Flies DB and Chen L. The new B7s: playing a pivotal role in tumor immunity. J. Immunother. 2007; 30:251-60. |
| Immunogen | Rabbit polyclonal VTCN1 antibody was raised against an 18 amino acid peptide near the center of human VTCN1. The immunogen is located within amino acids 70 - 120 of VTCN1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| VTCN1 antibody can be used for detection of VTCN1 by Western blot at 1 - 2 μg/mL. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | VTCN1 |
|---|---|
| Alternative Names | VTCN1 Antibody: B7X B7H4 B7S1 B7-H4 B7h.5 VCTN1 PRO1291 UNQ659/PRO1291 V-set domain-containing T-cell activation inhibitor 1 Immune costimulatory protein B7-H4 |
| Molecular Weight(MW) | Predicted: 31 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat: (83%) |
Application
Western blot analysis of VTCN1 in EL4 cell lysate with VTCN1 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1287 - EL4 Cell Lysate |
|---|---|
| Application Notes | VTCN1 antibody can be used for detection of VTCN1 by Western blot at 1 - 2 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | VTCN1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | VTCN1 Antibody is supplied in PBS containing 0.02% sodium azide. |
