WB
Western blot analysis of B7H4 on different lysates with Rabbit anti-B7H4 antibody at 1/1,000 dilution. Lane 1: MDA-MB-468 cell lysate (no heat) (15 µg/Lane), Lane 2: HeLa cell lysate (negative) (30 µg/Lane), Lane 3: NIH:OVCAR-3 cell lysate (30 µg/Lane), Lane 4: Mouse uterus tissue lysate (30 µg/Lane), Lane 5: Rat ovary tissue lysate (15 µg/Lane), Notice: no heat means the lysate is not boiled. Exposure time: 35 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of SK-Br-3 (positive) and HeLa (negative) labeling B7H4 with Rabbit anti-B7H4 antibody at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-B7H4 antibody at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.| Product Name | B7H4 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within human B7H4 aa 101-150 / 282. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:1000 ICC/IF:1:1000 |
|
| Species Reactivity | HumanMouseRabbit |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | VTCN1 |
|---|---|
| Gene Synonyms | B7X B7H4 B7S1 B7-H4 B7h.5 VCTN1 PRO1291 |
| Gene Full Name | V-set domain containing T cell activation inhibitor 1 |
| Gene Summary | This gene encodes a protein belonging to the B7 costimulatory protein family. Proteins in this family are present on the surface of antigen-presenting cells and interact with ligand bound to receptors on the surface of T cells. Studies have shown that high levels of the encoded protein has been correlated with tumor progression. A pseudogene of this gene is located on chromosome 20. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011] |
| Molecular Weight(MW) | 31kDa(Observed band size: 60-75kDa) |
| Cellular Localization | Cell membrane. |
| Entrez Gene | 79679 |
|---|
WB
Western blot analysis of B7H4 on different lysates with Rabbit anti-B7H4 antibody at 1/1,000 dilution. Lane 1: MDA-MB-468 cell lysate (no heat) (15 µg/Lane), Lane 2: HeLa cell lysate (negative) (30 µg/Lane), Lane 3: NIH:OVCAR-3 cell lysate (30 µg/Lane), Lane 4: Mouse uterus tissue lysate (30 µg/Lane), Lane 5: Rat ovary tissue lysate (15 µg/Lane), Notice: no heat means the lysate is not boiled. Exposure time: 35 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of SK-Br-3 (positive) and HeLa (negative) labeling B7H4 with Rabbit anti-B7H4 antibody at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-B7H4 antibody at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.| Application Notes | WB:1:1000 ICC/IF:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
