Application
Western blot analysis in CEM cell line lysates (35ug/lane).| Product Name | WNT3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Proto-oncogene Wnt-3, Proto-oncogene Int-4 homolog, WNT3, INT4 |
| Product description | The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98% amino acid identity to mouse Wnt3 protein, and 84% to human WNT3A protein, another WNT gene product. The mouse studies show the requirement of Wnt3 in primary axis formation in the mouse. Studies of the gene expression suggest that this gene may play a key role in some cases of human breast, rectal, lung, and gastric cancer through activation of the WNT-beta-catenin-TCF signaling pathway. This gene is clustered with WNT15, another family member, in the chromosome 17q21 region.1) Nikopensius, T., et al. Birth Defects Res. PartClin. Mol. Teratol. 88(9):748-756(2010) |
| Immunogen | This WNT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 155-184 amino acids from the Central region of human WNT3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | WNT3 |
|---|---|
| Alternative Names | Proto-oncogene Wnt-3 Proto-oncogene Int-4 homolog WNT3 INT4 |
| Molecular Weight(MW) | 40 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Chicken |
Application
Western blot analysis in CEM cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
