Application
Western blot analysis of YMEL1 Antibody in HepG2 cell line lysates (35ug/lane)Application
Western blot analysis of YMEL1 Antibody in CHO cell line lysates (35ug/lane)Application
YMEL1 Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | YMEL1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATP-dependent zinc metalloprotease YME1L1, 3424-, ATP-dependent metalloprotease FtsH1, Meg-4, Presenilin-associated metalloprotease, PAMP, YME1-like protein 1, YME1L1, FTSH1, YME1L |
| Product description | YMEL1 is the human ortholog of yeast mitochondrial AAA metalloprotease, Yme1p. It is localized in the mitochondria and can functionally complement a yme1 disruptant yeast strain. It is proposed that this gene plays a role in mitochondrial protein metabolism and could be involved in mitochondrial pathologies.1) Grupe, A., et al. Am. J. Hum. Genet. 78(1):78-88(2006) |
| Immunogen | This YMEL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 191-219 amino acids from the N-terminal region of human YMEL1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HamsterHuman |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | YME1L1 |
|---|---|
| Alternative Names | ATP-dependent zinc metalloprotease YME1L1 3424- ATP-dependent metalloprotease FtsH1 Meg-4 Presenilin-associated metalloprotease PAMP YME1-like protein 1 YME1L1 FTSH1 YME1L |
| Molecular Weight(MW) | 86 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western blot analysis of YMEL1 Antibody in HepG2 cell line lysates (35ug/lane)
Application
Western blot analysis of YMEL1 Antibody in CHO cell line lysates (35ug/lane)
Application
YMEL1 Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
