WB
Western blot analysis of YME1L1 using anti-YME1L1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-YME1L1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of YME1L1 using anti-YME1L1 antibody.YME1L1 was detected in a paraffin-embedded section of mouse heart tissue. The tissue section was incubated with rabbit anti-YME1L1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of YME1L1 using anti-YME1L1 antibody.YME1L1 was detected in a paraffin-embedded section of rat heart tissue. The tissue section was incubated with rabbit anti-YME1L1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
ICC/IF analysis of YME1L1 using anti-YME1L1 antibody.YME1L1 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-YME1L1 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).| Product Name | Rabbit polyclonal antibody to YME1L1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human YME1L1 recombinant protein (Position: Y402-L770). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | YME1L1 |
|---|---|
| Gene Synonyms | FTSH MEG4 PAMP OPA11 YME1L |
| Gene Full Name | YME1 like 1 ATPase |
| Gene Summary | The protein encoded by this gene is the human ortholog of yeast mitochondrial AAA metalloprotease, Yme1p. It is localized in the mitochondria and can functionally complement a yme1 disruptant yeast strain. It is proposed that this gene plays a role in mitochondrial protein metabolism and could be involved in mitochondrial pathologies. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011] |
| Alternative Names | FTSH FTSH1 Meg 4 MEG4 PAMP YME1 like protein 1 YME1L YME1L1 |
| Molecular Weight(MW) | 80 kDa(Observed band size: 63 kDa) |
| Cellular Localization | Membrane,Mitochondrion,Mitochondrion inner membrane |
WB
Western blot analysis of YME1L1 using anti-YME1L1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-YME1L1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of YME1L1 using anti-YME1L1 antibody.YME1L1 was detected in a paraffin-embedded section of mouse heart tissue. The tissue section was incubated with rabbit anti-YME1L1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of YME1L1 using anti-YME1L1 antibody.YME1L1 was detected in a paraffin-embedded section of rat heart tissue. The tissue section was incubated with rabbit anti-YME1L1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
ICC/IF analysis of YME1L1 using anti-YME1L1 antibody.YME1L1 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-YME1L1 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
