Application
Western blot analysis of ZBTB5 in mouse brain tissue lysate with ZBTB5 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of ZBTB5 in human brain tissue with ZBTB5 antibody at 20 μg/mL.Application
Immunohistochemistry of ZBTB5 in human brain tissue with ZBTB5 antibody at 2.5 μg/mL.| Product Name | ZBTB5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ZBTB5 Antibody: KIAA0354, Zinc finger and BTB domain-containing protein 5 |
| Product description | ZBTB5 Antibody: The ZBTB family of proteins is comprised of diverse zinc finger proteins that also contain a BTB (BR-C, ttk and bab) domain. ZBTB5 was identified though sequence analysis as a POZ domain Kruppel-like zinc finger (POK) protein. Further experiments indicated that it binds DNA and can directly repress transcription of the cell cycle arrest gene p21. ZBTB5 can also interact with co-repressor histone deacetylase complexes such as BCoR, NCoR, and SMRT via its POZ domain, resulting in deacetylation of histones Ac-H3 and Ac-H4 at the proximal promoter. ZBTB5 stimulates both cell proliferation and cell cycle progression, suggesting that it may act as a potential proto-oncogene.1) Strausberg RL, Feingold EA, Grouse LH, et al. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc. Natl. Acad. Sci. USA2002; 99:16899-903. |
| Immunogen | ZBTB5 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human ZBTB6. The immunogen is located within the last 50 amino acids of ZBTB5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| ZTBT5 antibody can be used for detection of ZTBT5 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ZBTB5 |
|---|---|
| Alternative Names | ZBTB5 Antibody: KIAA0354 Zinc finger and BTB domain-containing protein 5 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | At least two isoforms of ZBTB5 are known to exist; this antibody will recognize both isoforms. This antibody is predicted to not cross-react with other ZBTB protein family members. |
Application
Western blot analysis of ZBTB5 in mouse brain tissue lysate with ZBTB5 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of ZBTB5 in human brain tissue with ZBTB5 antibody at 20 μg/mL.
Application
Immunohistochemistry of ZBTB5 in human brain tissue with ZBTB5 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1403 - Mouse Brain Tissue Lysate |
|---|---|
| Application Notes | ZTBT5 antibody can be used for detection of ZTBT5 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ZBTB5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ZBTB5 Antibody is supplied in PBS containing 0.02% sodium azide. |
