WB
Positive control:A431,K562 and TM4 cell lysates .
Recommended dilution: 500-2000.
Gel: 6%SDS-PAGE .
Lysate: 40 μg .
Lane 1-3: A431,K562 and TM4 cel lysates .
Primary antibody: ZBTB5 Antibody at
dilution 1/400 .
Secondary antibody: Goat anti rabbit IgG at 1/8000
dilution .
Exposure time: 20 seconds .
IHC
Predicted cell location: Nucleus and Cytoplasm .
Positive control: Human gastric cancer .
Recommended dilution: 30-150 .
The image on the left is immunohistochemistry of paraffin-embedded
Human gastric cancer tissue using ZBTB5 Antibody at
dilution 1/40.