Application
Western blot analysis of ZEB1 in HeLa cell lysate with ZEB1 antibody at 1μg/mL.Application
Immunofluorescence of ZEB1 in Hela cells with ZEB1 antibody at 20 μg/mL.Application
Immunocytochemistry of ZEB1 in HeLa cells with ZEB1 antibody at 20 μg/mL.Application
Immunocytochemistry of ZEB1 in K562 cells with ZEB1 antibody at 2.5 μg/ml.Application
Immunohistochemistry of ZEB1 in human liver tissue with ZEB1 antibody at 5 μg/mL.| Product Name | ZEB1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | ZEB1 Antibody: ZEB1, initially identified as the delta-crystallin enhancer binding protein delta EF1, is a DNA-binding protein that binds to a modified E-box sequence and has been implicated in postgastrulation embryogenesis. ZEB1 binds to the promoter of several hemapoietic genes, including interleukin-2, CD4, GATA-3, and alpha-integrin, and mice in which ZEB1 has been targeted show thymic atrophy, and severe defects in lymphocyte differentiation. Recent evidence suggests that ZEB1 also regulates the accumulation of adipose tissue and may play a role in obesity. Mutations in this gene have been associated with late-onset Fuchs endothelial corneal dystrophy.1) Funahashi J, Sekido R, Murai K, et al. Delta-crystallin enhancer protein delta EF1 is a zinc finger-homeodomain protein implicated in postgastrulation embryogenesis. Dev.1993; 119:433-46. |
| Immunogen | ZEB1 antibody was raised against a 15 amino acid synthetic peptide near the center of human ZEB1. The immunogen is located within amino acids 530 - 580 of ZEB1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFIHC-PWB |
| ZEB1 antibody can be used for detection of ZEB1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL and immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ZEB1 |
|---|---|
| Alternative Names | ZEB1 Antibody: BZP TCF8 AREB6 FECD6 NIL2A PPCD3 ZFHEP ZFHX1A DELTAEF1 Zinc finger E-box-binding homeobox 1 NIL-2-A zinc finger protein TCF-8 |
| Molecular Weight(MW) | Predicted: 124 kDa Observed: 130 kDa |
| Sequence Similarities | 3) Cat. No. 17-001 - HeLa Cell Slide |
| Tissue Specificity | Multiple isoforms of ZEB1 are known to exist. |
Application
Western blot analysis of ZEB1 in HeLa cell lysate with ZEB1 antibody at 1μg/mL.
Application
Immunofluorescence of ZEB1 in Hela cells with ZEB1 antibody at 20 μg/mL.
Application
Immunocytochemistry of ZEB1 in HeLa cells with ZEB1 antibody at 20 μg/mL.
Application
Immunocytochemistry of ZEB1 in K562 cells with ZEB1 antibody at 2.5 μg/ml.
Application
Immunohistochemistry of ZEB1 in human liver tissue with ZEB1 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | ZEB1 antibody can be used for detection of ZEB1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL and immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | ZEB1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ZEB1 Antibody is supplied in PBS containing 0.02% sodium azide. |
