Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.| Product Name | ZNF496 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | ZNF496 contains a SCAN box, a KRAB-A domain, and four consensus C2H2-type zinc fingers preceded by a unique finger derivative, referred to herein as the C2HR motif. The C2HR motif functions to mediate protein-protein interaction with the cysteine-rich (C5HCH) domain of NSD1 in a Zn (II)-dependent fashion, and when tethered to RNA polymerase II promoters, represses transcription in an NSD1-dependent manner. ZNF496 contains a novel type of zinc finger motif that functions as a docking site for NSD1 and is more than just a degenerate evolutionary remnant of a C2H2 motif.1) Rual, J.F., (2005) Nature 437 (7062), 1173-1178. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ZNF496. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ZNF496 antibody can be used for detection of ZNF496 by ELISA at 1:62500. ZNF496 antibody can be used for detection of ZNF496 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | DogHumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ZNF496 |
|---|---|
| Alternative Names | ZNF496 NIZP1 ZFP496 ZSCAN49 ZKSCAN17 |
| Molecular Weight(MW) | 67 kDa |
Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | ZNF496 antibody can be used for detection of ZNF496 by ELISA at 1:62500. ZNF496 antibody can be used for detection of ZNF496 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store ZNF496 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
Data sheet for OM295905