Application
Fig1: Western blot analysis of HSP60 on different cell lysates using anti-HSP60 antibody at 1/2000 dilution. Positive control: Lane 1: Hela Lane 2: HepG2 Lane 3: Jurkat Lane 4: PC12 Lane 5: F9Application
Fig2: ICC staining HSP60 in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-HSP60 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-HSP60 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-HSP60 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-HSP60 antibody. Counter stained with hematoxylin.| Product Name | HSP60 [1-80] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 60 kDa chaperonin antibody 60 kDa heat shock protein, mitochondrial antibody CH60_HUMAN antibody Chaperonin 60 antibody Chaperonin, 60-KD antibody CPN60 antibody fa04a05 antibody GROEL antibody heat shock 60kDa protein 1 (chaperonin) antibody Heat shock protein 1 (chaperonin) antibody Heat shock protein 60 antibody Heat shock protein 65 antibody heat shock protein family D (Hsp60) member 1 antibody HLD4 antibody Hsp 60 antibody HSP 65 antibody HSP-60 antibody HSP60 antibody HSP65 antibody HSPD1 antibody HuCHA60 antibody Mitochondrial matrix protein P1 antibody P60 lymphocyte protein antibody short heat shock protein 60 Hsp60s1 antibody SPG14 antibody |
| Product description | The 60 kDa heat shock protein (HSP60) is a highly conserved protein that acts as a chaperone involved in correct folding of newly synthesized proteins. Mainly expressed in mitochondria, HPS60 has also been found in the cytosol of normal heart and muscle cells. The HSP60 proteins are abundant in the cell and are highly conserved between bacteria and human, with 50%-61% sequence homology. |
| Immunogen | peptide |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHC |
| WB:1:2,000 ICC:1:200 IHC:1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Alternative Names | 60 kDa chaperonin antibody
60 kDa heat shock protein mitochondrial antibody CH60_HUMAN antibody Chaperonin 60 antibody Chaperonin 60-KD antibody CPN60 antibody fa04a05 antibody GROEL antibody heat shock 60kDa protein 1 (chaperonin) antibody Heat shock protein 1 (chaperonin) antibody Heat shock protein 60 antibody Heat shock protein 65 antibody heat shock protein family D (Hsp60) member 1 antibody HLD4 antibody Hsp 60 antibody HSP 65 antibody HSP-60 antibody HSP60 antibody HSP65 antibody HSPD1 antibody HuCHA60 antibody Mitochondrial matrix protein P1 antibody P60 lymphocyte protein antibody short heat shock protein 60 Hsp60s1 antibody SPG14 antibody |
|---|---|
| Molecular Weight(MW) | 61kDa |
| Cellular Localization | Mitochondrion matrix |
| SwissProt ID | P10809 |
|---|
Application
Fig1: Western blot analysis of HSP60 on different cell lysates using anti-HSP60 antibody at 1/2000 dilution. Positive control: Lane 1: Hela Lane 2: HepG2 Lane 3: Jurkat Lane 4: PC12 Lane 5: F9
Application
Fig2: ICC staining HSP60 in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-HSP60 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-HSP60 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-HSP60 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-HSP60 antibody. Counter stained with hematoxylin.| Positive Control | Hela, HepG2, Jurkat, PC12, F9, human breast cancer tissue, human kidney tissue, mouse kidney tissue, mouse heart tissue. |
|---|---|
| Application Notes | WB:1:2,000 ICC:1:200 IHC:1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
