Application
Fig1: Western blot analysis of Vimentin on different cell lysates using anti- Vimentin antibody at 1/2000 dilution. Positive?control: Lane 1: A549 Lane 2: Hela Lane 3: NIH/3T3 Lane 4: Mouse embryonic stem cellApplication
Fig2: ICC staining vimentin in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human lung tissue using vimentin antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using vimentin antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using vimentin antibody. Counter stained with hematoxylin.| Product Name | Vimentin [D4-B11] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CTRCT30 antibody Epididymis luminal protein 113 antibody FLJ36605 antibody HEL113 antibody VIM antibody VIME_HUMAN antibody Vimentin antibody |
| Product description | Vimentin plays a significant role in supporting and anchoring the position of the organelles in the cytosol. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. In essence, vimentin is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. Vimentin has been shown to eliminate toxic proteins in JUNQ and IPOD inclusion bodies in asymmetric division of asymmetric division of mammalian cell lines. It has been used as a sarcoma tumor marker to identify mesenchyme. Methylation of the vimentin gene has been established as a biomarker of colon cancer and this is being utilized in the development of fecal tests for colon cancer. High levels of DNA methylation in the promotor region have also been associated with markedly decreased survival in hormone positive breast cancers. |
| Immunogen | peptide |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:2,000-1:5,000 ICC:1:200 IHC:1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Alternative Names | CTRCT30 antibody Epididymis luminal protein 113 antibody FLJ36605 antibody HEL113 antibody VIM antibody VIME_HUMAN antibody Vimentin antibody |
|---|---|
| Molecular Weight(MW) | 54 kDa |
| Cellular Localization | Cytoplasm |
| SwissProt ID | P08670 |
|---|
Application
Fig1: Western blot analysis of Vimentin on different cell lysates using anti- Vimentin antibody at 1/2000 dilution. Positive?control: Lane 1: A549 Lane 2: Hela Lane 3: NIH/3T3 Lane 4: Mouse embryonic stem cell
Application
Fig2: ICC staining vimentin in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human lung tissue using vimentin antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using vimentin antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using vimentin antibody. Counter stained with hematoxylin.| Positive Control | A549, Hela, NIH/3T3, mouse embryonic stem cell human lung?tissue, human colon carcinoma tissue, human stomach carcinoma tissue, mouse lung tissue |
|---|---|
| Application Notes | WB:1:2,000-1:5,000 ICC:1:200 IHC:1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
