Application
Fig1: Western blot analysis of Cytokeratin-7 on different cell lysates using anti- Cytokeratin-7 antibody at 1/1000 dilution. Positive?control: Lane 1: MCF-7 Lane 2: A549 Lane 3: Hela Lane 4: HepG2 Lane 5: SKBR3 Lane6: SKOV3 Lane 7: SW1990Application
Fig2: Immunohistochemical analysis of paraffin-embedded human lung?carcinoma tissue using anti-Cytokeratin-7 antibody. Counter stained with hematoxylin.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Cytokeratin-7 antibody. Counter stained with hematoxylin.| Product Name | Cytokeratin-7 [3-G3-D8] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Keratin-7 is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. Keratin-7 is found in simple glandular epithelia, and in transitional epithelium. Epithelial cells of the lung and breast both contain keratin-7, but some other glandular epithelia, such as those of the colon and prostate, do not. Because the keratin-7 antigen is found in both healthy and neoplastic cells, antibodies to CK7 can be used in immunohistochemistry to distinguish ovarian and transitional cell carcinomas from colonic and prostate cancers, respectively. |
| Immunogen | peptide |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WB,IHC |
| WB:1:1,000 IHC:1:200 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Purification | Unpurified |
| Alternative Names | CK 7 antibody
CK-7 antibody
CK7 antibody
Cytokeratin 7 antibody
Cytokeratin-7 antibody
D15Wsu77e antibody
K2C7 antibody
K2C7_HUMAN antibody
K7 antibody
Keratin 7 antibody
Keratin 7 type II antibody Keratin type II cytoskeletal 7 antibody Keratin 55K type II cytoskeletal antibody Keratin simple epithelial antibody Keratin simple epithelial type I K7 antibody Keratin type II cytoskeletal 7 antibody Keratin-7 antibody Krt2-7 antibody KRT7 antibody MGC11625 antibody MGC129731 antibody MGC3625 antibody Sarcolectin antibody SCL antibody Type II mesothelial keratin K7 antibody Type-II keratin Kb7 antibody |
|---|---|
| Molecular Weight(MW) | 51 kDa |
| Cellular Localization | Cytoplasm |
| SwissProt ID | P08729 |
|---|
Application
Fig1: Western blot analysis of Cytokeratin-7 on different cell lysates using anti- Cytokeratin-7 antibody at 1/1000 dilution. Positive?control: Lane 1: MCF-7 Lane 2: A549 Lane 3: Hela Lane 4: HepG2 Lane 5: SKBR3 Lane6: SKOV3 Lane 7: SW1990
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human lung?carcinoma tissue using anti-Cytokeratin-7 antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Cytokeratin-7 antibody. Counter stained with hematoxylin.| Positive Control | MCF-7, A549, Hela, HepG2, SKBR3, SKOV3, SW1990, human lung?carcinoma tissue, human breast tissue |
|---|---|
| Application Notes | WB:1:1,000 IHC:1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
