Application
Fig1: Western blot analysis of OCT-4 on different cell lysates using anti- OCT-4 antibody at 1/1000 dilution. Positive control: Line 1: NCCIT Line2 :D3Application
Fig2: ICC staining of OCT-4 in NCCIT cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining of OCT-4 in F9 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Product Name | OCT-4 [A3-C11] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Octamer binding transcription factor 4 antibody MGC22487 antibody Oct 3 antibody Oct 4 antibody Oct-3 antibody Oct-4 antibody OCT3 antibody Oct4 antibody Octamer binding protein 3 antibody Octamer binding protein 4 antibody Octamer binding transcription factor 3 antibody Octamer-binding protein 3 antibody Octamer-binding protein 4 antibody Octamer-binding transcription factor 3 antibody OTF 3 antibody OTF 4 antibody OTF-3 antibody OTF3 antibody OTF4 antibody PO5F1_HUMAN antibody POU class 5 homeobox 1 antibody POU domain class 5 transcription factor 1 antibody POU domain transcription factor OCT4 antibody POU domain, class 5, transcription factor 1 antibody POU-type homeodomain-containing DNA-binding protein antibody POU5F2 antibody |
| Product description | Oct-4 (POU5F1) is transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). It forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Oct-4 is critical for early embryogenesis and for embryonic stem cell pluripotency. It is expressed in developing brain and highest levels also found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. |
| Immunogen | peptide |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICC |
| WB:1:1,000-1:5,000 ICC:1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 2mg/ml |
| Alternative Names | Octamer binding transcription factor 4 antibody
MGC22487 antibody Oct 3 antibody Oct 4 antibody Oct-3 antibody Oct-4 antibody OCT3 antibody Oct4 antibody Octamer binding protein 3 antibody Octamer binding protein 4 antibody Octamer binding transcription factor 3 antibody Octamer-binding protein 3 antibody Octamer-binding protein 4 antibody Octamer-binding transcription factor 3 antibody OTF 3 antibody OTF 4 antibody OTF-3 antibody OTF3 antibody OTF4 antibody PO5F1_HUMAN antibody POU class 5 homeobox 1 antibody POU domain class 5 transcription factor 1 antibody POU domain transcription factor OCT4 antibody POU domain class 5 transcription factor 1 antibody POU-type homeodomain-containing DNA-binding protein antibody POU5F2 antibody |
|---|---|
| Molecular Weight(MW) | 39 kDa |
| Cellular Localization | Nucleus |
| SwissProt ID | Q01860 |
|---|
Application
Fig1: Western blot analysis of OCT-4 on different cell lysates using anti- OCT-4 antibody at 1/1000 dilution. Positive control: Line 1: NCCIT Line2 :D3
Application
Fig2: ICC staining of OCT-4 in NCCIT cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining of OCT-4 in F9 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Positive Control | NCCIT, D3, F9 |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
