Application
Fig1: Western blot analysis of EpCAM on different cell lysates using anti- EpCAM antibody at 1/1000 dilution.Positive control:Lane1: HelaLane2: NIH/3T3Lane3: MCF-7Application
Fig2: ICC staining EpCAM in D3 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining EpCAM in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using EpCAM antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using EpCAM antibody. Counter stained with hematoxylin.Application
Fig6: ICC staining EpCAM in SW1990 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EpCAM antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using EpCAM antibody. Counter stained withhematoxylin.| Product Name | EP-CAM [3-E1-E2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 17 1A antibody 323/A3 antibody Adenocarcinoma associated antigen antibody Adenocarcinoma-associated antigen antibody Antigen identified by monoclonal antibody AUA1 antibody AUA1 antibody CD326 antibody CD326 antigen antibody Cell surface glycoprotein Trop 1 antibody Cell surface glycoprotein Trop 2 antibody Cell surface glycoprotein Trop-1 antibody CO 17A antibody CO17 1A antibody CO17A antibody DIAR5 antibody EGP 2 antibody EGP antibody EGP2 antibody EGP314 antibody EGP40 antibody Ep CAM antibody Ep-CAM antibody EPCAM antibody EPCAM_HUMAN antibody EpCAM1 antibody Epithelial cell adhesion molecule antibody Epithelial Cell Adhesion Molecule Intracellular Domain (EpCAM-ICD) antibody Epithelial cell surface antigen antibody Epithelial cellular adhesion molecule antibody Epithelial glycoprotein 1 antibody Epithelial glycoprotein 314 antibody Epithelial glycoprotein antibody ESA antibody GA733 1 antibody GA733 2 antibody GA733-2 antibody gastrointestinal tumor-associated antigen 2, 35-KD glycoprotein antibody gp4 antibody hEGP 2 antibody hEGP314 antibody HNPCC8 antibody Human epithelial glycoprotein 2 antibody KS 1/4 antigen antibody KS1/4 antibody KSA antibody Ly74 antibody Lymphocyte antigen 74 antibody M1S 1 antibody M1S2 antibody M4S1 antibody Major gastrointestinal tumor associated protein GA733 2 antibody Major gastrointestinal tumor-associated protein GA733-2 antibody mEGP314 antibody Membrane component chromosome 4 surface marker (35kD glycoprotein) antibody Membrane component, chromosome 4, surface marker 1 antibody Membrane component, chromosome 4, surface marker antibody MIC18 antibody MK 1 antibody Protein 289A antibody TACD1 antibody TACSTD1 antibody TROP1 antibody Tumor associated calcium signal transducer 1 antibody Tumor associated calcium signal transducer 2 precursor antibody Tumor-associated calcium signal transducer 1 antibody |
| Product description | Epithelial cell adhesion molecule (EpCAM) is a transmembrane glycoprotein mediating Ca2+-independent homotypic cell-cell adhesion in epithelia. EpCAM is also involved in cell signaling, migration, proliferation, and differentiation. Additionally, EpCAM has oncogenic potential via its capacity to upregulate c-myc, e-fabp, and cyclins A & E. Since EpCAM is expressed exclusively in epithelia and epithelial-derived neoplasms, EpCAM can be used as diagnostic marker for various cancers. It appears to play a role in tumorigenesis and metastasis of carcinomas, so it can also act as a potential prognostic marker and as a potential target for immunotherapeutic strategies. Mutations in EpCAM have also been associated with congenital tufting enteropathy which causes intractable diarrhea in newborn children. Specificity/Source: This antibody is produced by immunizing mice with recombinant protein corresponding to a region of EpCAM. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:1,000-1:2,000 ICC:1:100-1:200 IHC:1:200-1:500 FC:1:100-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 2mg/ml |
| Alternative Names | 17 1A antibody
323/A3 antibody
Adenocarcinoma associated antigen antibody
Adenocarcinoma-associated antigen antibody
Antigen identified by monoclonal antibody
AUA1 antibody
AUA1 antibody
CD326 antibody
CD326 antigen antibody
Cell surface glycoprotein Trop 1 antibody
Cell surface glycoprotein Trop 2 antibody
Cell surface glycoprotein Trop-1 antibody
CO 17A antibody
CO17 1A antibody
CO17A antibody
DIAR5 antibody
EGP 2 antibody
EGP antibody
EGP2 antibody
EGP314 antibody
EGP40 antibody
Ep CAM antibody
Ep-CAM antibody
EPCAM antibody
EPCAM_HUMAN antibody
EpCAM1 antibody
Epithelial cell adhesion molecule antibody
Epithelial Cell Adhesion Molecule Intracellular Domain (EpCAM-ICD) antibody
Epithelial cell surface antigen antibody
Epithelial cellular adhesion molecule antibody
Epithelial glycoprotein 1 antibody
Epithelial glycoprotein 314 antibody
Epithelial glycoprotein antibody
ESA antibody
GA733 1 antibody
GA733 2 antibody
GA733-2 antibody
gastrointestinal tumor-associated antigen 2 35-KD glycoprotein antibody gp4 antibody hEGP 2 antibody hEGP314 antibody HNPCC8 antibody Human epithelial glycoprotein 2 antibody KS 1/4 antigen antibody KS1/4 antibody KSA antibody Ly74 antibody Lymphocyte antigen 74 antibody M1S 1 antibody M1S2 antibody M4S1 antibody Major gastrointestinal tumor associated protein GA733 2 antibody Major gastrointestinal tumor-associated protein GA733-2 antibody mEGP314 antibody Membrane component chromosome 4 surface marker (35kD glycoprotein) antibody Membrane component chromosome 4 surface marker 1 antibody Membrane component chromosome 4 surface marker antibody MIC18 antibody MK 1 antibody Protein 289A antibody TACD1 antibody TACSTD1 antibody TROP1 antibody Tumor associated calcium signal transducer 1 antibody Tumor associated calcium signal transducer 2 precursor antibody Tumor-associated calcium signal transducer 1 antibody |
|---|---|
| Molecular Weight(MW) | 35 kDa |
| Cellular Localization | Cell membrane |
| SwissProt ID | P16422 |
|---|
Application
Fig1: Western blot analysis of EpCAM on different cell lysates using anti- EpCAM antibody at 1/1000 dilution.Positive control:Lane1: HelaLane2: NIH/3T3Lane3: MCF-7
Application
Fig2: ICC staining EpCAM in D3 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining EpCAM in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using EpCAM antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using EpCAM antibody. Counter stained with hematoxylin.
Application
Fig6: ICC staining EpCAM in SW1990 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EpCAM antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using EpCAM antibody. Counter stained withhematoxylin.| Positive Control | Hela, NIH/3T3, MCF-7, A431, SW1990, human colon carcinoma tissue, human breast carcinoma tissue, human kidney tissue, mouse kidney tissue |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:100-1:200 IHC:1:200-1:500 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
