Application
Fig1: Western blot analysis of Bax on different lysates using anti-BAX antibody at 1/500 dilution. Positive?control: Lane1: Raji Lane2: Mouse lymphoid tissueApplication
Fig2: ICC staining BAX in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using BAX antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using BAX antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of Hela?cells with vimentin antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgA (FITC) was used as the secondary antibody.| Product Name | BAX [3-D2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | BAX is a member of the Bcl-2 gene family. Apoptosis regulator BAX promotes apoptosis by binding to and antagonizing the Bcl-2 protein. In healthy mammalian cells, the majority of BAX is found in the cytosol, but upon initiation of apoptotic signaling, Bax undergoes a conformational shift. Upon induction of apoptosis, BAX becomes organelle membrane-associated, and in particular, mitochondrial membrane associated. The expression of BAX is upregulated by the tumor suppressor protein p53, and BAX has been shown to be involved in p53-mediated apoptosis. The p53 protein is a transcription factor that, when activated as part of the cell's response to stress, regulates many downstream target genes, including BAX. |
| Immunogen | This antibody is produced by immunizing mice with a synthetic peptide (KLH-coupled) corresponding to N-terminal BAX. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgA |
| Host Species | Mouse |
| Tested Applications | WB,ICC,IHC,FC |
| WB:1:500 ICC:1:200 IHC:1:200 FC:1:50 |
|
| Species Reactivity | Human,Mouse |
| Concentration | 2mg/ml |
| Purification | Unpurified |
| Alternative Names | Apoptosis regulator BAX antibody BAX antibody Bax-protein antibody BAX_HUMAN antibody BAXA antibody Baxdelta2G9 antibody Baxdelta2G9omega antibody Baxdelta2omega antibody Bcl-2-like protein 4 antibody BCL2 associated X protein antibody BCL2 associated X protein omega antibody BCL2 associated X protein transcript variant delta2 antibody Bcl2-L-4 antibody BCL2L4 antibody membrane isoform alpha antibody |
|---|---|
| Molecular Weight(MW) | 21 kDa |
| Cellular Localization | Mitochondrion membrane, cytoplasm |
Application
Fig1: Western blot analysis of Bax on different lysates using anti-BAX antibody at 1/500 dilution. Positive?control: Lane1: Raji Lane2: Mouse lymphoid tissue
Application
Fig2: ICC staining BAX in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using BAX antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using BAX antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of Hela?cells with vimentin antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgA (FITC) was used as the secondary antibody.| Positive Control | Raji, mouse lymphoid tissue, HepG2, human gallbladder tissue, mouse testis tissue, Hela |
|---|---|
| Application Notes | WB:1:500 ICC:1:200 IHC:1:200 FC:1:50 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
