Application
Fig1: Western blot analysis of GFAP on different cell lysates using anti- GFAP antibody at 1/5000 dilution. Positive control: Line 1: Rat brain Line2 :Human brainApplication
Fig2: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-GFAP antibody. Counter stained with hematoxylin.Application
Fig3: ICC staining of GFAP in A172 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining of GFAP in N2A cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Flow cytometric analysis of Hela cells with GFAP antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.| Product Name | GFAP [1-D4] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | wu:fb34h11 antibody ALXDRD antibody cb345 antibody etID36982.3 antibody FLJ42474 antibody FLJ45472 antibody GFAP antibody GFAP_HUMAN antibody gfapl antibody Glial fibrillary acidic protein antibody Intermediate filament protein antibody wu:fk42c12 antibody xx:af506734 antibody zgc:110485 antibody |
| Product description | GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. In particular, vimentin filaments are present at early developmental stages, while GFAP filaments are characteristic of differentiated and mature brain astrocytes. In addition, GFAP intermediate filaments are also present in nonmyelin-forming Schwann cells in the peripheral nervous system. |
| Immunogen | peptide |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHCICCFC |
| WB:1:2,000-1:5,000 ICC:1:200: IHC:1:200 FC:1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Alternative Names | wu:fb34h11 antibody ALXDRD antibody cb345 antibody etID36982.3 antibody FLJ42474 antibody FLJ45472 antibody GFAP antibody GFAP_HUMAN antibody gfapl antibody Glial fibrillary acidic protein antibody Intermediate filament protein antibody wu:fk42c12 antibody xx:af506734 antibody zgc:110485 antibody |
|---|---|
| Molecular Weight(MW) | 50 kDa |
| Cellular Localization | Cytoplasm, intermediate filament |
| SwissProt ID | P14136 |
|---|
Application
Fig1: Western blot analysis of GFAP on different cell lysates using anti- GFAP antibody at 1/5000 dilution. Positive control: Line 1: Rat brain Line2 :Human brain
Application
Fig2: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-GFAP antibody. Counter stained with hematoxylin.
Application
Fig3: ICC staining of GFAP in A172 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining of GFAP in N2A cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Flow cytometric analysis of Hela cells with GFAP antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.| Positive Control | Mouse brain tissue, rat brain tissue, human brain tissue,A172 ,N2A ,Hela. |
|---|---|
| Application Notes | WB:1:2,000-1:5,000 ICC:1:200: IHC:1:200 FC:1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
