Application
Fig1: Flow cytometric analysis of Jurkat cells with PD1 blocking antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).Application
Fig2: Blockade of Receptor-ligand Interaction at the cellular level using PD1 transfected CHO cells. In a functional ELISA, 50ng of PD1 antibody will block 52% of the binding of PD-L1-Fc to PD1 transfected CHO cells.| Product Name | PD1 Blocking [3A11] |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFFC |
| ElISA:1:1,000-1:5,000 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Molecular Weight(MW) | 32 kDa |
|---|---|
| Cellular Localization | Membrane. |
| SwissProt ID | Q15116 |
|---|
Application
Fig1: Flow cytometric analysis of Jurkat cells with PD1 blocking antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Application
Fig2: Blockade of Receptor-ligand Interaction at the cellular level using PD1 transfected CHO cells. In a functional ELISA, 50ng of PD1 antibody will block 52% of the binding of PD-L1-Fc to PD1 transfected CHO cells.| Positive Control | Jurkat. |
|---|---|
| Application Notes | ElISA:1:1,000-1:5,000 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
